Store-operated Ca Entry Facilitates the Lipopolysaccharide-induced Cyclooxygenase-2 Expression in Gastric Cancer Cells

Overview

Journal Sci Rep

Specialty Science

Date 2017 Oct 18

PMID 29038542

Citations 11

Authors

Jhen-Hong Wong

Kuo-Hao Ho

Sean Nam

Wen-Li Hsu

Chia-Hsien Lin

Che-Mai Chang

Jaw-Yuan Wang

Wei-Chiao Chang

Affiliations

Soon will be listed here.

Abstract

Helicobacter pylori has been identified as one of the major causes of chronic gastritis, gastric and duodenal ulcers, and gastric cancer. Lipopolysaccharide (LPS) is a major component of the outer membrane of gram-negative bacteria, and H. pylori LPS might play an exclusively important role in activating inflammatory pathways in monocytes and macrophages. To study the role of LPS in the underlying mechanism of inflammatory responses, we established an in vitro model using the human AGS gastric cancer cell line. We found that LPS mediates inflammation through setting off a cascade of events: activation of the store-operated calcium (SOC) channel, initiation of downstream NF-κB signaling, and phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2). Phosphorylated ERK1/2 promotes the nuclear translocation of NF-κB, and eventually elevates the expression level of COX-2, a major inflammatory gene.

Citing Articles

Strategies of in evading host innate and adaptive immunity: insights and prospects for therapeutic targeting.

Fan J, Zhu J, Xu H Front Cell Infect Microbiol. 2024; 14:1342913.

PMID: 38469348 PMC: 10925771. DOI: 10.3389/fcimb.2024.1342913.

Targeting Store-Operated Calcium Entry Regulates the Inflammation-Induced Proliferation and Migration of Breast Cancer Cells.

Alqinyah M, Alhamed A, Alnefaie H, Algahtani M, Badr A, Albogami A Biomedicines. 2023; 11(6).

PMID: 37371732 PMC: 10296208. DOI: 10.3390/biomedicines11061637.

Blockade of store-operated calcium entry sensitizes breast cancer cells to cisplatin therapy via modulating inflammatory response.

Alhamed A, Alqinyah M, Alsufayan M, Alhaydan I, Alassmrry Y, Alnefaie H Saudi Pharm J. 2023; 31(2):245-254.

PMID: 36942275 PMC: 10023550. DOI: 10.1016/j.jsps.2022.12.009.

NMDARs antagonist MK801 suppresses LPS-induced apoptosis and mitochondrial dysfunction by regulating subunits of NMDARs via the CaM/CaMKII/ERK pathway.

Han W, Hao X, Hong Y, Zhao S, Chen X, Wang R Cell Death Discov. 2023; 9(1):59.

PMID: 36774369 PMC: 9922289. DOI: 10.1038/s41420-023-01362-9.

L-Type Ca Channel Inhibition Rescues the LPS-Induced Neuroinflammatory Response and Impairments in Spatial Memory and Dendritic Spine Formation.

Kim J, Jeon S, Jeong H, Park H, Kim J, Hoe H Int J Mol Sci. 2022; 23(21).

PMID: 36362394 PMC: 9655622. DOI: 10.3390/ijms232113606.

References

Hu Y, Fang J, Xiao S . Can the incidence of gastric cancer be reduced in the new century?. J Dig Dis. 2012; 14(1):11-5. DOI: 10.1111/j.1751-2980.2012.00647.x. View

Wang D, DuBois R . The role of COX-2 in intestinal inflammation and colorectal cancer. Oncogene. 2009; 29(6):781-8. PMC: 3181054. DOI: 10.1038/onc.2009.421. View

Moen E, Wen S, Anwar T, Cross-Knorr S, Brilliant K, Birnbaum F . Regulation of RKIP function by Helicobacter pylori in gastric cancer. PLoS One. 2012; 7(5):e37819. PMC: 3360604. DOI: 10.1371/journal.pone.0037819. View

Luigiano C, Ferrara F, Fagoonee S, Fabbri C, Cennamo V, Virgilio C . Is Helicobacter pylori the infectious target to prevent gastric cancer? An interdisciplinary point of view. Infect Disord Drug Targets. 2012; 12(5):340-5. DOI: 10.2174/187152612804142206. View

Huang W, Chai C, Chen W, Hou M, Wang Y, Chiu Y . Histamine regulates cyclooxygenase 2 gene activation through Orai1-mediated NFκB activation in lung cancer cells. Cell Calcium. 2011; 50(1):27-35. DOI: 10.1016/j.ceca.2011.04.004. View

Miller T . Protective effects of prostaglandins against gastric mucosal damage: current knowledge and proposed mechanisms. Am J Physiol. 1983; 245(5 Pt 1):G601-23. DOI: 10.1152/ajpgi.1983.245.5.G601. View

Franchi A, Di Girolamo G, de los Santos A, Marti M, Gimeno M . Effects of lysine clonixinate on cyclooxygenase I and II in rat lung and stomach preparations. Prostaglandins Leukot Essent Fatty Acids. 1999; 58(6):421-4. DOI: 10.1016/s0952-3278(98)90164-8. View

Simmons D, Botting R, Hla T . Cyclooxygenase isozymes: the biology of prostaglandin synthesis and inhibition. Pharmacol Rev. 2004; 56(3):387-437. DOI: 10.1124/pr.56.3.3. View

Hynes S, Ferris J, Szponar B, Wadstrom T, Fox J, ORourke J . Comparative chemical and biological characterization of the lipopolysaccharides of gastric and enterohepatic helicobacters. Helicobacter. 2004; 9(4):313-23. DOI: 10.1111/j.1083-4389.2004.00237.x. View

10.

EMMONS P, Hampton J, Harrison M, HONOUR A, Mitchell J . Effect of prostaglandin E1 on platelet behaviour in vitro and in vivo. Br Med J. 1967; 2(5550):468-72. PMC: 1842033. DOI: 10.1136/bmj.2.5550.468. View

11.

Bhandari A, Crowe S . Helicobacter pylori in gastric malignancies. Curr Gastroenterol Rep. 2012; 14(6):489-96. DOI: 10.1007/s11894-012-0296-y. View

12.

Subbaramaiah K, Dannenberg A . Cyclooxygenase 2: a molecular target for cancer prevention and treatment. Trends Pharmacol Sci. 2003; 24(2):96-102. DOI: 10.1016/S0165-6147(02)00043-3. View

13.

Sun L, Carpenter G . Epidermal growth factor activation of NF-kappaB is mediated through IkappaBalpha degradation and intracellular free calcium. Oncogene. 1998; 16(16):2095-102. DOI: 10.1038/sj.onc.1201731. View

14.

Aggarwal B, Gehlot P . Inflammation and cancer: how friendly is the relationship for cancer patients?. Curr Opin Pharmacol. 2009; 9(4):351-69. PMC: 2730981. DOI: 10.1016/j.coph.2009.06.020. View

15.

Baggiolini M, Clark-Lewis I . Interleukin-8, a chemotactic and inflammatory cytokine. FEBS Lett. 1992; 307(1):97-101. DOI: 10.1016/0014-5793(92)80909-z. View

16.

Chen L, Chen B, Chang W . Activating protein 1-mediated cyclooxygenase-2 expression is independent of N-terminal phosphorylation of c-Jun. Mol Pharmacol. 2005; 67(6):2057-69. DOI: 10.1124/mol.104.010900. View

17.

Chuah S, Tsay F, Hsu P, Wu D . A new look at anti-Helicobacter pylori therapy. World J Gastroenterol. 2011; 17(35):3971-5. PMC: 3199554. DOI: 10.3748/wjg.v17.i35.3971. View

18.

Hanson P, Moran A, Butler K . Paracellular permeability is increased by basal lipopolysaccharide in a primary culture of colonic epithelial cells; an effect prevented by an activator of Toll-like receptor-2. Innate Immun. 2010; 17(3):269-82. DOI: 10.1177/1753425910367813. View

19.

Soler M, Camacho M, Escudero J, Iniguez M, Vila L . Human vascular smooth muscle cells but not endothelial cells express prostaglandin E synthase. Circ Res. 2000; 87(6):504-7. DOI: 10.1161/01.res.87.6.504. View

20.

Lonigro A, Itskovitz H, Crowshaw K, McGiff J . Dependency of renal blood flow on prostaglandin synthesis in the dog. Circ Res. 1973; 32(6):712-7. DOI: 10.1161/01.res.32.6.712. View