Glutathione Peroxidase Activity, Plasma Total Antioxidant Capacity, and Urinary F2- Isoprostanes As Markers of Oxidative Stress in Anemic Dogs

Overview

Journal J Vet Intern Med

Specialties General Medicine
Veterinary Medicine

Date 2017 Oct 15

PMID 29031029

Citations 12

Authors

A Kendall

A Woolcock

A Brooks

G E Moore

Affiliations

Soon will be listed here.

Abstract

Background: Oxidative stress plays a role in the pathophysiology of several diseases and has been documented as a contributor to disease in both the human and veterinary literature. One at-risk cell is the erythrocyte, however, the role of oxidative stress in anemia in dogs has not been widely investigated.

Hypothesis/objective: Anemic dogs will have an alteration in the activity of glutathione peroxidase (GPx), a decrease in of total antioxidant capacity (TAC), and an increased concentration of urinary 15-F -isoprostanes (F -IsoP) when compared to healthy dogs.

Animals: 40 client-owned dogs with anemia (PCV <30%) age-matched to 40 client-owned healthy control dogs.

Methods: Prospective, cross-sectional study. Whole blood GPx activity, plasma TAC, and urinary F -isoprostane concentrations were evaluated in each dog and compared between groups.

Results: Anemic dogs had significantly lower GPx activity (43.1 × 10 +/- 1.6 × 10 U/L) than did dogs in the control group (75.8 × 10 +/- 2.0 × 10 U/L; P < 0.0001). The GPx activity in dogs with hemolysis (10 +/- 0.8 × 10 U/L) was not significantly different (P = 0.57) than in dogs with nonhemolytic anemia (43.5 × 10 +/- 1.1 × 10 U/L). The TAC concentrations (P = 0.15) and urinary F -isoprostanes (P = 0.73) did not significantly differ between groups.

Conclusions And Clinical Importance: Glutathione peroxidase activity was significantly decreased in anemic dogs indicating oxidative stress. Additional studies are warranted to determine if antioxidant supplementation would improve survival and overall outcome as part of a therapeutic regimen for anemic dogs.

Citing Articles

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References

Freeman L, Rush J, Milbury P, Blumberg J . Antioxidant status and biomarkers of oxidative stress in dogs with congestive heart failure. J Vet Intern Med. 2005; 19(4):537-41. DOI: 10.1892/0891-6640(2005)19[537:asaboo]2.0.co;2. View

Morrow J, Roberts L . The isoprostanes: unique bioactive products of lipid peroxidation. Prog Lipid Res. 1997; 36(1):1-21. DOI: 10.1016/s0163-7827(97)00001-5. View

Viviano K, VanderWielen B . Effect of N-acetylcysteine supplementation on intracellular glutathione, urine isoprostanes, clinical score, and survival in hospitalized ill dogs. J Vet Intern Med. 2013; 27(2):250-8. DOI: 10.1111/jvim.12048. View

Viviano K, Lavergne S, Goodman L, VanderWielen B, Grundahl L, Padilla M . Glutathione, cysteine, and ascorbate concentrations in clinically ill dogs and cats. J Vet Intern Med. 2009; 23(2):250-7. DOI: 10.1111/j.1939-1676.2008.0238.x. View

Koren E, Kohen R, Ginsburg I . Polyphenols enhance total oxidant-scavenging capacities of human blood by binding to red blood cells. Exp Biol Med (Maywood). 2010; 235(6):689-99. DOI: 10.1258/ebm.2010.009370. View

Freeman L, Brown D, Rush J . Assessment of degree of oxidative stress and antioxidant concentrations in dogs with idiopathic dilated cardiomyopathy. J Am Vet Med Assoc. 1999; 215(5):644-6. View

Hesta M, Ottermans C, Krammer-Lukas S, Zentek J, Hellweg P, Buyse J . The effect of vitamin C supplementation in healthy dogs on antioxidative capacity and immune parameters. J Anim Physiol Anim Nutr (Berl). 2009; 93(1):26-34. DOI: 10.1111/j.1439-0396.2007.00774.x. View

Milne G, Sanchez S, Musiek E, Morrow J . Quantification of F2-isoprostanes as a biomarker of oxidative stress. Nat Protoc. 2007; 2(1):221-6. DOI: 10.1038/nprot.2006.375. View

Pesillo S, Freeman L, Rush J . Assessment of lipid peroxidation and serum vitamin E concentration in dogs with immune-mediated hemolytic anemia. Am J Vet Res. 2005; 65(12):1621-4. DOI: 10.2460/ajvr.2004.65.1621. View

10.

Silva A, de Almeida B, Soeiro C, Ferreira W, de Lima V, Ciarlini P . Oxidative stress, superoxide production, and apoptosis of neutrophils in dogs with chronic kidney disease. Can J Vet Res. 2013; 77(2):136-41. PMC: 3605930. View

11.

Sagols E, Priymenko N . Oxidative stress in dog with heart failure: the role of dietary Fatty acids and antioxidants. Vet Med Int. 2011; 2011:180206. PMC: 3087355. DOI: 10.4061/2011/180206. View

12.

Paglia D, Valentine W . Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967; 70(1):158-69. View

13.

Plevnik Kapun A, Salobir J, Levart A, Tavcar Kalcher G, Nemec Svete A, Kotnik T . Vitamin E supplementation in canine atopic dermatitis: improvement of clinical signs and effects on oxidative stress markers. Vet Rec. 2014; 175(22):560. DOI: 10.1136/vr.102547. View

14.

Waggiallah H, Alzohairy M . The effect of oxidative stress on human red cells glutathione peroxidase, glutathione reductase level, and prevalence of anemia among diabetics. N Am J Med Sci. 2012; 3(7):344-7. PMC: 3336886. DOI: 10.4297/najms.2011.3344. View

15.

Young I . Measurement of total antioxidant capacity. J Clin Pathol. 2001; 54(5):339. PMC: 1731415. DOI: 10.1136/jcp.54.5.339. View

16.

MACDOUGALL L . Red cell metabolism in iron deficiency anemia. 3. The relationship between glutathione peroxidase, catalase, serum vitamin E, and susceptibility of iron-deficient red cells to oxidative hemolysis. J Pediatr. 1972; 80(5):775-82. DOI: 10.1016/s0022-3476(72)80130-6. View

17.

Steinberg M, BRAUER M, NECHELES T . Acute hemolytic anemia associated with erythrocyte glutathione-peroxidase deficiency. Arch Intern Med. 1970; 125(2):302-3. View

18.

Du W, Adam Z, Rani R, Zhang X, Pang Q . Oxidative stress in Fanconi anemia hematopoiesis and disease progression. Antioxid Redox Signal. 2008; 10(11):1909-21. PMC: 2695607. DOI: 10.1089/ars.2008.2129. View

19.

Pandey K, Rizvi S . Biomarkers of oxidative stress in red blood cells. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2011; 155(2):131-6. DOI: 10.5507/bp.2011.027. View

20.

Chen T, Gionfriddo J, Tai P, Novakowski A, Alyahya K, Madl J . Oxidative stress increases in retinas of dogs in acute glaucoma but not in chronic glaucoma. Vet Ophthalmol. 2014; 18(4):261-70. DOI: 10.1111/vop.12177. View