Methylated DNA-binding Protein is Present in Various Mammalian Cell Types

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1988 Aug 25

PMID 2901711

Citations 15

Authors

P C Supakar

D Weist

D L Zhang

N Inamdar

X Y Zhang

R Khan

K C Ehrlich

M Ehrlich

Affiliations

Soon will be listed here.

Abstract

A DNA-binding protein from human placenta, methylated DNA-binding protein (MDBP), binds to certain DNA sequences only when they contain 5-methylcytosine (m5C) residues at specific positions. We found a very similar DNA-binding activity in nuclear extracts of rat tissues, calf thymus, human embryonal carcinoma cells, HeLa cells, and mouse LTK cells. Like human placental MDBP, the analogous DNA-binding proteins from the above mammalian cell lines formed a number of different low-electrophoretic-mobility complexes with a 14-bp MDBP-specific oligonucleotide duplex. All of these complexes exhibited the same DNA methylation specificity and DNA sequence specificity. From the extracts of rat and calf tissues, oligonucleotide protein complexes formed that also had the same specificity as human placental MDBP although they had a higher electrophoretic mobility probably due to digestion by proteases in the nuclear extracts. Although MDBP activity was found in various mammalian cell types, it was not detected in extracts of cultured mosquito cells and so may be associated only with cells with vertebrate-type DNA methylation.

Citing Articles

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A preference of histone H1 for methylated DNA.

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The major histocompatibility complex class II promoter-binding protein RFX (NF-X) is a methylated DNA-binding protein.

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