Immunocytochemical and Ultrastructural Characterization of Human T-lymphotropic Virus Type I (HTLV-I)-producing Rabbit Lymphoid Cell Lines

Overview

Journal Arch Virol

Specialty Microbiology

Date 1988 Jan 1

PMID 2899999

Citations 1

Authors

Y Ohtsuki

I Miyoshi

T Oka

K Hayashi

K Takahashi

M Furihata

J Iwata

T Takeuchi

H Sonobe

Affiliations

Soon will be listed here.

Abstract

Fine structural and immunocytochemical characterization of rabbit lymphoid cell lines transformed by human T-lymphotropic virus type I (HTLV-I) was carried out. All nine cell lines tested were reactive with anti-HTLV-I-positive human, monkey, and rabbit sera and monoclonal antibody to HTLV-Ip 19, but not with anti-HTLV-I-negative sera and monoclonal antibodies to human Ia and pan-T antigens. All cell lines were strongly positive for monoclonal antibodies to rabbit Ia and pan-T antigens. Ultrastructurally, each cell line contained C-type virus particles in varying numbers in the extracellular space. These particles showed replication patterns similar to those in HTLV-I or simian T-lymphotropic virus type I (STLV-I)-producing human or monkey cells. In addition, anti-HTLV-I-positive rabbit serum gave positive immunoreactivity to HTLV-I or STLV-I by indirect immunoferritin method. These results indicate that the ultramorphology and replication patterns of HTLV-I in rabbit cell lines are indistinguishable from those of HTLV-I in human and monkey cell lines, HTLV-I in rabbit cells shares the common surface antigenic determinants with HTLV-I or STLV-I in human or monkey cells, and that these cells are definitely rabbit T cells bearing their own Ia antigens.

Citing Articles

Suppressive effects of interferons on the production and release of human T-lymphotropic virus type-I (HTLV-I).

Oka T, Ohtsuki Y, Sonobe H, Furihata M, Miyoshi I Arch Virol. 1990; 115(1-2):63-73.

PMID: 1701080 DOI: 10.1007/BF01310623.

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