The Clinical Application of MicroRNAs in Infectious Disease

Overview

Journal Front Immunol

Specialty Allergy & Immunology

Date 2017 Oct 11

PMID 28993774

Citations 82

Authors

Ruth E Drury

Daniel OConnor

Andrew J Pollard

Affiliations

Soon will be listed here.

Abstract

MicroRNAs (miRNAs) are short single-stranded non-coding RNA sequences that posttranscriptionally regulate up to 60% of protein encoding genes. Evidence is emerging that miRNAs are key mediators of the host response to infection, predominantly by regulating proteins involved in innate and adaptive immune pathways. miRNAs can govern the cellular tropism of some viruses, are implicated in the resistance of some individuals to infections like HIV, and are associated with impaired vaccine response in older people. Not surprisingly, pathogens have evolved ways to undermine the effects of miRNAs on immunity. Recognition of this has led to new experimental treatments, RG-101 and Miravirsen-hepatitis C treatments which target host miRNA. miRNAs are being investigated as novel infection biomarkers, and they are being used to design attenuated vaccines, e.g., against Dengue virus. This comprehensive review synthesizes current knowledge of miRNA in host response to infection with emphasis on potential clinical applications, along with an evaluation of the challenges still to be overcome.

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References

Tay H, Kaiko G, Plank M, Li J, Maltby S, Essilfie A . Antagonism of miR-328 increases the antimicrobial function of macrophages and neutrophils and rapid clearance of non-typeable Haemophilus influenzae (NTHi) from infected lung. PLoS Pathog. 2015; 11(4):e1004549. PMC: 4404141. DOI: 10.1371/journal.ppat.1004549. View

Vickers K, Palmisano B, Shoucri B, Shamburek R, Remaley A . MicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteins. Nat Cell Biol. 2011; 13(4):423-33. PMC: 3074610. DOI: 10.1038/ncb2210. View

Heiss B, Maximova O, Pletnev A . Insertion of microRNA targets into the flavivirus genome alters its highly neurovirulent phenotype. J Virol. 2010; 85(4):1464-72. PMC: 3028900. DOI: 10.1128/JVI.02091-10. View

Cheong J, Shin H, Kim Y, Cho S . Association of polymorphism in MicroRNA 219-1 with clearance of hepatitis B virus infection. J Med Virol. 2013; 85(5):808-14. DOI: 10.1002/jmv.23551. View

Bai X, Nicot C . miR-28-3p is a cellular restriction factor that inhibits human T cell leukemia virus, type 1 (HTLV-1) replication and virus infection. J Biol Chem. 2015; 290(9):5381-90. PMC: 4342455. DOI: 10.1074/jbc.M114.626325. View

Yen Y, Huang C, Wei K, Wang J, Lu S, Lee C . MicroRNA-122 as a predictor of HBsAg seroclearance in hepatitis B and C dual infected patients treated with interferon and ribavirin. Sci Rep. 2016; 6:33816. PMC: 5036026. DOI: 10.1038/srep33816. View

He L, Hannon G . MicroRNAs: small RNAs with a big role in gene regulation. Nat Rev Genet. 2004; 5(7):522-31. DOI: 10.1038/nrg1379. View

Chugh P, Damania B, Dittmer D . Toll-like receptor-3 is dispensable for the innate microRNA response to West Nile virus (WNV). PLoS One. 2014; 9(8):e104770. PMC: 4134228. DOI: 10.1371/journal.pone.0104770. View

Teng Y, Miao J, Shen X, Yang X, Wang X, Ren L . The modulation of MiR-155 and MiR-23a manipulates Klebsiella pneumoniae Adhesion on Human pulmonary Epithelial cells via Integrin α5β1 Signaling. Sci Rep. 2016; 6:31918. PMC: 4989230. DOI: 10.1038/srep31918. View

10.

Ni B, Rajaram M, Lafuse W, Landes M, Schlesinger L . Mycobacterium tuberculosis decreases human macrophage IFN-γ responsiveness through miR-132 and miR-26a. J Immunol. 2014; 193(9):4537-47. DOI: 10.4049/jimmunol.1400124. View

11.

Lee S, Song J, Kim S, Kim J, Hong Y, Kim Y . Selective degradation of host MicroRNAs by an intergenic HCMV noncoding RNA accelerates virus production. Cell Host Microbe. 2013; 13(6):678-90. DOI: 10.1016/j.chom.2013.05.007. View

12.

Rupp L, Brady B, Carpenter A, De Obaldia M, Bhandoola A, Bosselut R . The microRNA biogenesis machinery modulates lineage commitment during αβ T cell development. J Immunol. 2014; 193(8):4032-42. PMC: 4185242. DOI: 10.4049/jimmunol.1401359. View

13.

Zhou H, Rigoutsos I . MiR-103a-3p targets the 5' UTR of GPRC5A in pancreatic cells. RNA. 2014; 20(9):1431-9. PMC: 4138326. DOI: 10.1261/rna.045757.114. View

14.

Liu M, John C, Jarvis G . Induction of endotoxin tolerance by pathogenic Neisseria is correlated with the inflammatory potential of lipooligosaccharides and regulated by microRNA-146a. J Immunol. 2014; 192(4):1768-77. DOI: 10.4049/jimmunol.1301648. View

15.

Essandoh K, Li Y, Huo J, Fan G . MiRNA-Mediated Macrophage Polarization and its Potential Role in the Regulation of Inflammatory Response. Shock. 2016; 46(2):122-31. PMC: 4949115. DOI: 10.1097/SHK.0000000000000604. View

16.

Ellis-Connell A, Iempridee T, Xu I, Mertz J . Cellular microRNAs 200b and 429 regulate the Epstein-Barr virus switch between latency and lytic replication. J Virol. 2010; 84(19):10329-43. PMC: 2937814. DOI: 10.1128/JVI.00923-10. View

17.

Wang X, Gu H, Qin D, Yang L, Huang W, Essandoh K . Exosomal miR-223 Contributes to Mesenchymal Stem Cell-Elicited Cardioprotection in Polymicrobial Sepsis. Sci Rep. 2015; 5:13721. PMC: 4562230. DOI: 10.1038/srep13721. View

18.

Li J, Dai X, Zhang W, Sun S, Zeng Y, Zhao G . Upregulation of microRNA-146a by hepatitis B virus X protein contributes to hepatitis development by downregulating complement factor H. mBio. 2015; 6(2). PMC: 4453536. DOI: 10.1128/mBio.02459-14. View

19.

Lee Y, Kim M, Han J, Yeom K, Lee S, Baek S . MicroRNA genes are transcribed by RNA polymerase II. EMBO J. 2004; 23(20):4051-60. PMC: 524334. DOI: 10.1038/sj.emboj.7600385. View

20.

Ramos T, Sanchez-Abarca L, Muntion S, Preciado S, Puig N, Lopez-Ruano G . MSC surface markers (CD44, CD73, and CD90) can identify human MSC-derived extracellular vesicles by conventional flow cytometry. Cell Commun Signal. 2016; 14:2. PMC: 4709865. DOI: 10.1186/s12964-015-0124-8. View