Microtubule Stabilization Drives 3D Centrosome Migration to Initiate Primary Ciliogenesis

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2017 Oct 11

PMID 28993469

Citations 37

Authors

Amandine Pitaval

Fabrice Senger

Gaelle Letort

Xavier Gidrol

Laurent Guyon

James Sillibourne

Manuel Thery

Affiliations

Soon will be listed here.

Abstract

Primary cilia are sensory organelles located at the cell surface. Their assembly is primed by centrosome migration to the apical surface, yet surprisingly little is known about this initiating step. To gain insight into the mechanisms driving centrosome migration, we exploited the reproducibility of cell architecture on adhesive micropatterns to investigate the cytoskeletal remodeling supporting it. Microtubule network densification and bundling, with the transient formation of an array of cold-stable microtubules, and actin cytoskeleton asymmetrical contraction participate in concert to drive apical centrosome migration. The distal appendage protein Cep164 appears to be a key actor involved in the cytoskeleton remodeling and centrosome migration, whereas intraflagellar transport 88's role seems to be restricted to axoneme elongation. Together, our data elucidate the hitherto unexplored mechanism of centrosome migration and show that it is driven by the increase and clustering of mechanical forces to push the centrosome toward the cell apical pole.

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