Longitudinal Monitoring of Antibody Responses Against Tumor Cells Using Magneto-nanosensors with a Nanoliter of Blood

Overview

Journal Nano Lett

Publisher American Chemical Society

Specialty Biotechnology

Date 2017 Oct 10

PMID 28990786

Citations 8

Authors

Jung-Rok Lee

Carmel T Chan

Daniel Ruderman

Hui-Yen Chuang

Richard S Gaster

Michelle Atallah

Parag Mallick

Scott W Lowe

Sanjiv S Gambhir

Shan X Wang

Affiliations

Soon will be listed here.

Abstract

Each immunoglobulin isotype has unique immune effector functions. The contribution of these functions in the elimination of pathogens and tumors can be determined by monitoring quantitative temporal changes in isotype levels. Here, we developed a novel technique using magneto-nanosensors based on the effect of giant magnetoresistance (GMR) for longitudinal monitoring of total and antigen-specific isotype levels with high precision, using as little as 1 nL of serum. Combining in vitro serologic measurements with in vivo imaging techniques, we investigated the role of the antibody response in the regression of firefly luciferase (FL)-labeled lymphoma cells in spleen, kidney, and lymph nodes in a syngeneic Burkitt's lymphoma mouse model. Regression status was determined by whole body bioluminescent imaging (BLI). The magneto-nanosensors revealed that anti-FL IgG2a and total IgG2a were elevated and sustained in regression mice compared to non-regression mice (p < 0.05). This platform shows promise for monitoring immunotherapy, vaccination, and autoimmunity.

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References

Wang X, Yu J, Sreekumar A, Varambally S, Shen R, Giacherio D . Autoantibody signatures in prostate cancer. N Engl J Med. 2005; 353(12):1224-35. DOI: 10.1056/NEJMoa051931. View

Gaster R, Hall D, Nielsen C, Osterfeld S, Yu H, Mach K . Matrix-insensitive protein assays push the limits of biosensors in medicine. Nat Med. 2009; 15(11):1327-32. PMC: 4165514. DOI: 10.1038/nm.2032. View

Smith M . Rituximab (monoclonal anti-CD20 antibody): mechanisms of action and resistance. Oncogene. 2003; 22(47):7359-68. DOI: 10.1038/sj.onc.1206939. View

Paweletz C, Charboneau L, Bichsel V, Simone N, Chen T, Gillespie J . Reverse phase protein microarrays which capture disease progression show activation of pro-survival pathways at the cancer invasion front. Oncogene. 2001; 20(16):1981-9. DOI: 10.1038/sj.onc.1204265. View

Ito K, Smith B, Parashurama N, Yoon J, Song S, Miething C . Unexpected dissemination patterns in lymphoma progression revealed by serial imaging within a murine lymph node. Cancer Res. 2012; 72(23):6111-8. PMC: 3664177. DOI: 10.1158/0008-5472.CAN-12-2579. View

Sullivan S, Koutsonanos D, del Pilar Martin M, Lee J, Zarnitsyn V, Choi S . Dissolving polymer microneedle patches for influenza vaccination. Nat Med. 2010; 16(8):915-20. PMC: 2917494. DOI: 10.1038/nm.2182. View

Nimmerjahn F, Ravetch J . Divergent immunoglobulin g subclass activity through selective Fc receptor binding. Science. 2005; 310(5753):1510-2. DOI: 10.1126/science.1118948. View

Tran P, Peiffer D, Shin Y, Meek L, Brody J, Cho K . Microarray optimizations: increasing spot accuracy and automated identification of true microarray signals. Nucleic Acids Res. 2002; 30(12):e54. PMC: 117296. DOI: 10.1093/nar/gnf053. View

Lee J, Choi J, Shultz T, Wang S . Small Molecule Detection in Saliva Facilitates Portable Tests of Marijuana Abuse. Anal Chem. 2016; 88(15):7457-61. DOI: 10.1021/acs.analchem.6b01688. View

10.

Jeon Y, Choi Y, Kang J, Kim C, Jeong J, Lee D . Immune response to firefly luciferase as a naked DNA. Cancer Biol Ther. 2007; 6(5):781-6. DOI: 10.4161/cbt.6.5.4005. View

11.

Schmitt C, McCurrach M, de Stanchina E, Wallace-Brodeur R, Lowe S . INK4a/ARF mutations accelerate lymphomagenesis and promote chemoresistance by disabling p53. Genes Dev. 1999; 13(20):2670-7. PMC: 317110. DOI: 10.1101/gad.13.20.2670. View

12.

Zhong L, Coe S, Stromberg A, Khattar N, Jett J, Hirschowitz E . Profiling tumor-associated antibodies for early detection of non-small cell lung cancer. J Thorac Oncol. 2007; 1(6):513-9. View

13.

Lee J, Haddon D, Wand H, Price J, Diep V, Hall D . Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus. Sci Rep. 2016; 6:27623. PMC: 4899742. DOI: 10.1038/srep27623. View

14.

Scofield R . Autoantibodies as predictors of disease. Lancet. 2004; 363(9420):1544-6. DOI: 10.1016/S0140-6736(04)16154-0. View

15.

Hall D, Gaster R, Lin T, Osterfeld S, Han S, Murmann B . GMR biosensor arrays: a system perspective. Biosens Bioelectron. 2010; 25(9):2051-7. PMC: 2855856. DOI: 10.1016/j.bios.2010.01.038. View

16.

Bechstein D, Lee J, Ooi C, Gani A, Kim K, Wilson R . High performance wash-free magnetic bioassays through microfluidically enhanced particle specificity. Sci Rep. 2015; 5:11693. PMC: 4485157. DOI: 10.1038/srep11693. View

17.

Lee J, Bechstein D, Ooi C, Patel A, Gaster R, Ng E . Magneto-nanosensor platform for probing low-affinity protein-protein interactions and identification of a low-affinity PD-L1/PD-L2 interaction. Nat Commun. 2016; 7:12220. PMC: 4961847. DOI: 10.1038/ncomms12220. View

18.

Nakaya H, Wrammert J, Lee E, Racioppi L, Marie-Kunze S, Nicholas Haining W . Systems biology of vaccination for seasonal influenza in humans. Nat Immunol. 2011; 12(8):786-95. PMC: 3140559. DOI: 10.1038/ni.2067. View

19.

Stevens T, Bossie A, Sanders V, Fernandez-Botran R, Coffman R, Mosmann T . Regulation of antibody isotype secretion by subsets of antigen-specific helper T cells. Nature. 1988; 334(6179):255-8. DOI: 10.1038/334255a0. View

20.

Reynolds S, Muwonga J . OraQuick ADVANCE Rapid HIV-1/2 antibody test. Expert Rev Mol Diagn. 2004; 4(5):587-91. DOI: 10.1586/14737159.4.5.587. View