Genetic Transformation of Pseudomonas Phaseolicola by R-factor DNA
Overview
Molecular Biology
Authors
Affiliations
The bacterium Pseudomonas phaseolicola was successfully transformed, for the first time, with R-factors RSF1010 and pBR322 DNA by a calcium-shock and heat-pulse technique. Frequency of transformation for RSF1010 ranged from 0.8 x 10(-7) to 3.1 x 10(-6) and was ca. 0.4 x 10(-8) for pBR322.
Genetic transformation of Rhodopseudomonas sphaeroides by plasmid DNA.
Fornari C, Kaplan S J Bacteriol. 1982; 152(1):89-97.
PMID: 6981642 PMC: 221378. DOI: 10.1128/jb.152.1.89-97.1982.
cDNA cloning of portions of the bacteriophage phi 6 genome.
Mindich L, MacKenzie G, Strassman J, McGraw T, Metzger S, Romantschuk M J Bacteriol. 1985; 162(3):992-9.
PMID: 3858275 PMC: 215873. DOI: 10.1128/jb.162.3.992-999.1985.