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» Articles » PMID: 28989826

Isolation and Characterization of 30 Microsatellite Loci for (Taxodiaceae)

Overview
Journal Appl Plant Sci
Date 2017 Oct 10
PMID 28989826
Authors
Si-Si Wang
Yang Zhang
De-Chen Liu
Xiao-Wei Sun
Rong Wang
Yuan-Yuan Li
Affiliations
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Abstract

Premise Of The Study: To quantify the population-level genetic characteristics of (Taxodiaceae), an important timber conifer, we developed 30 pairs of microsatellite primers based on the nuclear genome.

Methods And Results: Using the streptavidin-biotin capture system, we developed 14 polymorphic and 16 monomorphic microsatellites. Polymorphisms were detected in 14 loci using 94 individual trees that were collected from three populations in Hubei and Zhejiang provinces and in Chongqing Municipality, China. There were three to 30 alleles per locus, and the observed and expected heterozygosities ranged from 0.0313-0.8333 and from 0.0313-0.9246, respectively. Cross-species amplification showed that two to seven polymorphic loci were functional in three of the five related species that were collected.

Conclusions: Our newly developed microsatellite primers provide neutral molecular markers that are beneficial to future studies of population genetics and germplasm conservation of .

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