Detection of Treponema Phagedenis-like Antibodies in Serum and Bulk Milk from Cows with and Without Digital Dermatitis

Overview

Journal J Vet Diagn Invest

Specialty Veterinary Medicine

Date 2017 Oct 8

PMID 28985709

Citations 6

Authors

Jenny Frossling

Anna Rosander

Camilla Bjorkman

Katarina Naslund

Marit Pringle

Affiliations

Soon will be listed here.

Abstract

Digital dermatitis (DD) in dairy cows is a widespread disease linked to infection with Treponema. The traditional diagnostic method is clinical inspection, which is subjective and laborious. We explored the performance of 4 different immunogenic proteins from Treponema phagedenis in a new antibody ELISA for analysis of serum or milk. Analysis of samples from 390 cows in 25 herds showed that the ELISA could distinguish the majority of cows with DD from healthy cows. By changing the cutoff and applying parallel or serial testing, high sensitivity or specificity could be achieved. The investigation indicated that aggregated test results can be useful in the assessment of a herd's DD status. In addition, analysis of bulk tank milk samples showed good agreement with results from individual cows. The test system could be useful in research on the epidemiology and immunology of DD.

Citing Articles

Assessment of immunological response to digital dermatitis pathogen derived antigens following infection, recovery, and reinfection.

Coatney J, Krull A, Gorden P, Shearer J, Humphrey S, Olsen S Front Vet Sci. 2024; 11:1487316.

PMID: 39711796 PMC: 11660806. DOI: 10.3389/fvets.2024.1487316.

Digital dermatitis in Swedish dairy herds assessed by ELISA targeting Treponema phagedenis in bulk tank milk.

Roelofs L, Frossling J, Rosander A, Bjerketorp J, Arabi Belaghi R, Hansson I BMC Vet Res. 2024; 20(1):168.

PMID: 38698418 PMC: 11064309. DOI: 10.1186/s12917-024-04021-y.

An In-House ELISA for Treponema Antibodies in Bulk Milk as Part of a Monitoring Tool for Claw Health in Dairy Herds.

Holzhauer M, Mars J, Holstege M, van der Heijden H Vet Sci. 2023; 10(9).

PMID: 37756093 PMC: 10537149. DOI: 10.3390/vetsci10090571.

A case-control study regarding factors associated with digital dermatitis in Norwegian dairy herds.

Ahlen L, Holmoy I, Nodtvedt A, Sogstad A, Fjeldaas T Acta Vet Scand. 2022; 64(1):19.

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Diagnosis of Bovine Digital Dermatitis: Exploring the Usefulness of Indirect ELISA.

Sucena Afonso J, Oikonomou G, Carter S, Clough H, Griffiths B, Rushton J Front Vet Sci. 2021; 8:728691.

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