Hispolon Suppresses Metastasis Via Autophagic Degradation of Cathepsin S in Cervical Cancer Cells

Overview

Journal Cell Death Dis

Specialties Cell Biology
General Medicine

Date 2017 Oct 6

PMID 28981104

Citations 36

Authors

Min-Chieh Hsin

Yi-Hsien Hsieh

Po-Hui Wang

Jiunn-Liang Ko

I-Lun Hsin

Shun-Fa Yang

Affiliations

Soon will be listed here.

Abstract

Hispolon, a phenolic compound isolated from Phellinus igniarius, induces apoptosis and anti-tumor effects in cancers. However, the molecular mechanism involved in hispolon-mediated tumor-suppressing activities observed in cervical cancer is poorly characterized. Here, we demonstrated that treatment with hispolon inhibited cell metastasis in two cervical cancer cell lines. In addition, the downregulation of the lysosomal protease Cathepsin S (CTSS) was critical for hispolon-mediated suppression of tumor cell metastasis in both in vitro and in vivo models. Moreover, hispolon induced autophagy, which increased LC3 conversion and acidic vesicular organelle formation. Mechanistically, hispolon inhibited the cell motility of cervical cells through the extracellular signal-regulated kinase (ERK) pathway, and blocking of the ERK pathway reversed autophagy-mediated cell motility and CTSS inhibition. Our results indicate that autophagy is essential for decreasing CTSS activity to inhibit tumor metastasis by hispolon treatment in cervical cancer; this finding provides a new perspective on molecular regulation.

Citing Articles

Cathepsins and their role in gynecological cancers: Evidence from two-sample Mendelian randomization analysis.

Li X, Sun L, Wu X, Qiu M, Ma X Medicine (Baltimore). 2025; 104(10):e41653.

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Enhancement hispolon production from Phellinus linteus via epigenetic-modified culture to inhibit human breast cancer cells.

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Methoxyhispolon Methyl Ether, a Hispolon Analog, Thwarts the SRC/STAT3/BCL-2 Axis to Provoke Human Triple-Negative Breast Cancer Cell Apoptosis In Vitro.

Liao C, Hsieh Y, Lu C, Dai W, Yang W, Cheng K Biomedicines. 2023; 11(10).

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Hispolon induces apoptosis in oral squamous cell carcinoma cells through JNK/HO-1 pathway activation.

Yang W, Chen Y, Su C, Chen M, Yeh C, Chen Y J Cell Mol Med. 2023; 27(9):1250-1260.

PMID: 36967712 PMC: 10148051. DOI: 10.1111/jcmm.17729.

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