Simultaneous LC-MS/MS Analysis of Eicosanoids and Related Metabolites in Human Serum, Sputum and BALF

Overview

Journal Biomed Chromatogr

Publisher Wiley

Specialties Biology
Chemistry

Date 2017 Oct 5

PMID 28975688

Citations 12

Authors

Rhishikesh Thakare

Yashpal S Chhonker

Nagsen Gautam

Amy Nelson

Richard Casaburi

Gerard Criner

Mark T Dransfield

Barry Make

Kendra K Schmid

Stephen I Rennard

Yazen Alnouti

Affiliations

Soon will be listed here.

Abstract

The differences among individual eicosanoids in eliciting different physiological and pathological responses are largely unknown because of the lack of valid and simple analytical methods for the quantification of individual eicosanoids and their metabolites in serum, sputum and bronchial alveolar lavage fluid (BALF). Therefore, a simple and sensitive LC-MS/MS method for the simultaneous quantification of 34 eicosanoids in human serum, sputum and BALF was developed and validated. This method is valid and sensitive with a limit of quantification ranging from 0.2 to 3 ng/mL for the various analytes, and has a large dynamic range (500 ng/mL) and a short run time (25 min). The intra- and inter-day accuracy and precision values met the acceptance criteria according to US Food and Drug Administration guidelines. Using this method, detailed eicosanoid profiles were quantified in serum, sputum and BALF from a pilot human study. In summary, a reliable and simple LC-MS/MS method to quantify major eicosanoids and their metabolites was developed and applied to quantify eicosanoids in human various fluids, demonstrating its suitability to assess eicosanoid biomarkers in human clinical trials.

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