Multifocus Structured Illumination Microscopy for Fast Volumetric Super-resolution Imaging

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2017 Oct 3

PMID 28966852

Citations 19

Authors

Sara Abrahamsson

Hans Blom

Ana Agostinho

Daniel C Jans

Aurelie Jost

Marcel Muller

Linnea Nilsson

Kristoffer Bernhem

Talley J Lambert

Rainer Heintzmann

Hjalmar Brismar

Affiliations

Soon will be listed here.

Abstract

We here report for the first time the synergistic implementation of structured illumination microscopy (SIM) and multifocus microscopy (MFM). This imaging modality is designed to alleviate the problem of insufficient volumetric acquisition speed in super-resolution biological imaging. SIM is a wide-field super-resolution technique that allows imaging with visible light beyond the classical diffraction limit. Employing multifocus diffractive optics we obtain simultaneous wide-field 3D imaging capability in the SIM acquisition sequence, improving volumetric acquisition speed by an order of magnitude. Imaging performance is demonstrated on biological specimens.

Citing Articles

Bi-Plane Multicolor Scanning Illumination Microscopy with Multispot Excitation and a Distorted Diffraction Grating.

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Fast volumetric multifocus structured illumination microscopy of subcellular dynamics in living cells.

Senftleben M, Bajor A, Hirata E, Abrahamsson S, Brismar H Biomed Opt Express. 2024; 15(4):2281-2292.

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Superresolution structured illumination microscopy reconstruction algorithms: a review.

Chen X, Zhong S, Hou Y, Cao R, Wang W, Li D Light Sci Appl. 2023; 12(1):172.

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Fluorescence Microscopy: a statistics-optics perspective.

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