(Myrtaceae) Fruits Protect HEPG2 Cells Against Carbon Tetrachloride-induced Toxicity

Overview

Journal Toxicol Rep

Date 2017 Oct 1

PMID 28962350

Citations 10

Authors

Thais de Oliveira Fernandes

Renato Ivan de Avila

Soraia Santana de Moura

Gerlon de Almeida Ribeiro

Maria Margareth Veloso Naves

Marize Campos Valadares

Affiliations

Soon will be listed here.

Abstract

(Myrtaceae) is an antioxidant compounds-rich Brazilian fruit popularly known as . In view of this, it was evaluated the hepatoprotective effects of pulp (GPE) or peel/seed (GPSE) hydroalcoholic extracts of on injured liver-derived HepG2 cells by CCl (4 mM). The results showed the presence of total phenolic in GPSE was (60%) higher when compared to GPE, associated with interesting antioxidant activity using DPPH• assay. Additionally, HPLC chromatograms and thin layer chromatography of GPE and GPSE showed the presence of flavonoids. Pretreatment of HepG2 cells with GPE or GPSE (both at 800-1000 μg/mL) significantly ( < 0.0001) protected against cytotoxicity induced by CCl Additionally, the cells treated with both extracts (both at 1000 μg/mL) showed normal morphology (general and nuclear) contrasting with apoptotic characteristics in the cells only exposed to CCl. In these experiments, GPSE also was more effective than GPE. In addition, CCl induced a marked increase in AST ( < 0.05) and ALT ( < 0.0001) levels, while GPE or GPSE significantly ( < 0.0001) reduced these levels, reaching values found in the control group. In conclusion, the results suggest that fruits exert hepatoprotective effects on HepG2 cells against the CCl-induced toxicity, probably, at least in part, associated with the presence of antioxidant compounds, especially flavonoids.

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References

Martin M, Ramos S, Mateos R, Serrano A, Izquierdo-Pulido M, Bravo L . Protection of human HepG2 cells against oxidative stress by cocoa phenolic extract. J Agric Food Chem. 2008; 56(17):7765-72. DOI: 10.1021/jf801744r. View

Boone L, Meyer D, Cusick P, Ennulat D, Bolliger A, Everds N . Selection and interpretation of clinical pathology indicators of hepatic injury in preclinical studies. Vet Clin Pathol. 2005; 34(3):182-8. DOI: 10.1111/j.1939-165x.2005.tb00041.x. View

Deb D, Parimala G, Saravana Devi S, Chakrabarti T . Role of Carum copticum seeds in modulating chromium-induced toxicity on human bronchial epithelial cells and human peripheral blood lymphocytes. Exp Toxicol Pathol. 2011; 64(7-8):889-97. DOI: 10.1016/j.etp.2011.03.012. View

Guo X, Liang B, Wang X, Fan F, Jin J, Lan R . Glycyrrhizic acid attenuates CCl₄-induced hepatocyte apoptosis in rats via a p53-mediated pathway. World J Gastroenterol. 2013; 19(24):3781-91. PMC: 3699029. DOI: 10.3748/wjg.v19.i24.3781. View

Wang S, Kao M, Wu S, Lo D, Wu J, Chang J . Oral administration of Trapa taiwanensis Nakai fruit skin extracts conferring hepatoprotection from CCl4-caused injury. J Agric Food Chem. 2011; 59(8):3686-92. DOI: 10.1021/jf1048386. View

Goya L, Mateos R, Bravo L . Effect of the olive oil phenol hydroxytyrosol on human hepatoma HepG2 cells. Protection against oxidative stress induced by tert-butylhydroperoxide. Eur J Nutr. 2007; 46(2):70-8. DOI: 10.1007/s00394-006-0633-8. View

Ha H, Shin H, Feitelson M, Yu D . Oxidative stress and antioxidants in hepatic pathogenesis. World J Gastroenterol. 2010; 16(48):6035-43. PMC: 3012582. DOI: 10.3748/wjg.v16.i48.6035. View

Porubsky P, Meneely K, Scott E . Structures of human cytochrome P-450 2E1. Insights into the binding of inhibitors and both small molecular weight and fatty acid substrates. J Biol Chem. 2008; 283(48):33698-707. PMC: 2586265. DOI: 10.1074/jbc.M805999200. View

Alia M, Ramos S, Mateos R, Granado-Serrano A, Bravo L, Goya L . Quercetin protects human hepatoma HepG2 against oxidative stress induced by tert-butyl hydroperoxide. Toxicol Appl Pharmacol. 2005; 212(2):110-8. DOI: 10.1016/j.taap.2005.07.014. View

10.

Scapagnini G, Vasto S, Sonya V, Abraham N, Nader A, Caruso C . Modulation of Nrf2/ARE pathway by food polyphenols: a nutritional neuroprotective strategy for cognitive and neurodegenerative disorders. Mol Neurobiol. 2011; 44(2):192-201. PMC: 5554938. DOI: 10.1007/s12035-011-8181-5. View

11.

Ozerkan D, Ozsoy N, Yilmaz E . Vitamin D and melatonin protect the cell's viability and ameliorate the CCl4 induced cytotoxicity in HepG2 and Hep3B hepatoma cell lines. Cytotechnology. 2014; 67(6):995-1002. PMC: 4628931. DOI: 10.1007/s10616-014-9738-8. View

12.

Fernandez-Panchon M, Villano D, Troncoso A, Garcia-Parrilla M . Antioxidant activity of phenolic compounds: from in vitro results to in vivo evidence. Crit Rev Food Sci Nutr. 2008; 48(7):649-71. DOI: 10.1080/10408390701761845. View

13.

Manibusan M, Odin M, Eastmond D . Postulated carbon tetrachloride mode of action: a review. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. 2007; 25(3):185-209. DOI: 10.1080/10590500701569398. View

14.

Noh J, Gang G, Kim Y, Yang K, Hwang J, Lee H . Antioxidant effects of the chestnut (Castanea crenata) inner shell extract in t-BHP-treated HepG2 cells, and CCl4- and high-fat diet-treated mice. Food Chem Toxicol. 2010; 48(11):3177-83. DOI: 10.1016/j.fct.2010.08.018. View

15.

Jacobo-Velazquez D, Cisneros-Zevallos L . Correlations of antioxidant activity against phenolic content revisited: a new approach in data analysis for food and medicinal plants. J Food Sci. 2010; 74(9):R107-13. DOI: 10.1111/j.1750-3841.2009.01352.x. View

16.

Shin S, Yang J, Ki S . Role of the Nrf2-ARE pathway in liver diseases. Oxid Med Cell Longev. 2013; 2013:763257. PMC: 3665261. DOI: 10.1155/2013/763257. View

17.

Ignat I, Volf I, Popa V . A critical review of methods for characterisation of polyphenolic compounds in fruits and vegetables. Food Chem. 2014; 126(4):1821-35. DOI: 10.1016/j.foodchem.2010.12.026. View

18.

Pareek A, Godavarthi A, Issarani R, Nagori B . Antioxidant and hepatoprotective activity of Fagonia schweinfurthii (Hadidi) Hadidi extract in carbon tetrachloride induced hepatotoxicity in HepG2 cell line and rats. J Ethnopharmacol. 2013; 150(3):973-81. DOI: 10.1016/j.jep.2013.09.048. View

19.

de Souza J, Piccinelli A, Aquino D, de Souza V, Schmitz W, Traesel G . Toxicological analysis and antihyperalgesic, antidepressant, and anti-inflammatory effects of Campomanesia adamantium fruit barks. Nutr Neurosci. 2014; 20(1):23-31. DOI: 10.1179/1476830514Y.0000000145. View

20.

Ramaiah S . A toxicologist guide to the diagnostic interpretation of hepatic biochemical parameters. Food Chem Toxicol. 2007; 45(9):1551-7. DOI: 10.1016/j.fct.2007.06.007. View