Relationship Between Antibiotic Susceptibility and Genotype in Clinical Isolates

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2017 Sep 30

PMID 28959242

Citations 23

Authors

Bing Li

Shiyi Yang

Haiqing Chu

Zhemin Zhang

Weijia Liu

LiuLin Luo

Wei Ma

Xiaogang Xu

Affiliations

Soon will be listed here.

Abstract

This study aimed to determine the antibiotic susceptibility and resistance related genotypes of . One hundred sixty-two clinical isolates were collected. Genomic data were obtained by whole genome sequencing. Single nucleotide polymorphism (SNP) analysis was conducted using the NCBI GenBank database and BLAST algorithm. The following genes were of interest: and . was further divided into 3 sequevars: , and M type [ with deletions in nucleotides 64 and 65, or 159 through 432]. Antibiotic susceptibility was assessed at 3 days (early reading time, ERT) and 14 days (late reading time, LRT) after clarithromycin (CLA) treatment. Three patterns of CLA resistance were observed. (1) Fifty-five (acquired resistance) isolates [45 , 1 and 9 M type] exhibited MIC ≥8 mg/L at ERT; among these isolates, 10 had an 2058/2059 mutation. (2) Sixty-two and 2 (induced resistance) isolates exhibited MIC ≤4 mg/L at ERT, but ≥8 mg/L at LRT. (3) Forty-three (sensitive and intermediate) isolates [14 , 1 , and 28 M type] exhibited MIC ≤4 mg/L at both ERT and LRT. No 1408 mutation or other meaningful SNP was found in 3 amikacin-resistant isolates. No correlation was found between or and susceptibility to the 8 other antibiotics tested. The and genotypes could predict the CLA resistance of clinical isolates. China has a large number of CLA-resistant isolates with sequevar. Treatment of infections should be based upon a comprehensive consideration of factors that include genotype and geographic location.

Citing Articles

The gene is responsible for intrinsic resistance to various drugs and virulence in by regulating cell division.

Ju Y, Li L, Zhang J, Yusuf B, Zeng S, Fang C Antimicrob Agents Chemother. 2024; 69(2):e0043324.

PMID: 39699214 PMC: 11823648. DOI: 10.1128/aac.00433-24.

TBAJ-587, a novel diarylquinoline, is active against .

Fan J, Tan Z, He S, Li A, Jia Y, Li J Antimicrob Agents Chemother. 2024; 68(12):e0094524.

PMID: 39470202 PMC: 11619292. DOI: 10.1128/aac.00945-24.

Analysis of Clinical Isolation Characteristics of Nontuberculous Mycobacteria and Drug Sensitivity of Rapidly Growing Mycobacteria in the General Hospital of Guangzhou, China.

Liu X, Lin Z, Li Y, Zhong Z, Wu A, Jiang Y Infect Drug Resist. 2024; 17:4079-4088.

PMID: 39319037 PMC: 11420897. DOI: 10.2147/IDR.S465468.

Whole-Genome Sequencing and Drug-Susceptibility Analysis of Serial Isolates from Thai Patients.

Kaewprasert O, Nonghanphithak D, Chetchotisakd P, Namwat W, Ong R, Faksri K Biology (Basel). 2022; 11(9).

PMID: 36138798 PMC: 9495349. DOI: 10.3390/biology11091319.

A Novel Leucyl-tRNA Synthetase Inhibitor, MRX-6038, Expresses Anti-Mycobacterium abscessus Activity and .

Wu W, He S, Li A, Guo Q, Tan Z, Liu S Antimicrob Agents Chemother. 2022; 66(9):e0060122.

PMID: 35969055 PMC: 9487484. DOI: 10.1128/aac.00601-22.

References

Lee S, Yoo H, Kim S, Koh W, Kim C, Park Y . The drug resistance profile of Mycobacterium abscessus group strains from Korea. Ann Lab Med. 2014; 34(1):31-7. PMC: 3885770. DOI: 10.3343/alm.2014.34.1.31. View

Hoefsloot W, van Ingen J, Andrejak C, Angeby K, Bauriaud R, Bemer P . The geographic diversity of nontuberculous mycobacteria isolated from pulmonary samples: an NTM-NET collaborative study. Eur Respir J. 2013; 42(6):1604-13. DOI: 10.1183/09031936.00149212. View

Olivier K, Griffith D, Eagle G, McGinnis 2nd J, Micioni L, Liu K . Randomized Trial of Liposomal Amikacin for Inhalation in Nontuberculous Mycobacterial Lung Disease. Am J Respir Crit Care Med. 2016; 195(6):814-823. PMC: 5363966. DOI: 10.1164/rccm.201604-0700OC. View

de Carvalho N, Sato D, Pavan F, Ferrazoli L, Chimara E . Resazurin Microtiter Assay for Clarithromycin Susceptibility Testing of Clinical Isolates of Mycobacterium abscessus Group. J Clin Lab Anal. 2016; 30(5):751-5. PMC: 6806693. DOI: 10.1002/jcla.21933. View

Mougari F, Amarsy R, Veziris N, Bastian S, Brossier F, Bercot B . Standardized interpretation of antibiotic susceptibility testing and resistance genotyping for Mycobacterium abscessus with regard to subspecies and erm41 sequevar. J Antimicrob Chemother. 2016; 71(8):2208-12. DOI: 10.1093/jac/dkw130. View

Brown-Elliott B, Vasireddy S, Vasireddy R, Iakhiaeva E, Howard S, Nash K . Utility of sequencing the erm(41) gene in isolates of Mycobacterium abscessus subsp. abscessus with low and intermediate clarithromycin MICs. J Clin Microbiol. 2015; 53(4):1211-5. PMC: 4365201. DOI: 10.1128/JCM.02950-14. View

Harada T, Akiyama Y, Kurashima A, Nagai H, Tsuyuguchi K, Fujii T . Clinical and microbiological differences between Mycobacterium abscessus and Mycobacterium massiliense lung diseases. J Clin Microbiol. 2012; 50(11):3556-61. PMC: 3486228. DOI: 10.1128/JCM.01175-12. View

Nash K, Brown-Elliott B, Wallace Jr R . A novel gene, erm(41), confers inducible macrolide resistance to clinical isolates of Mycobacterium abscessus but is absent from Mycobacterium chelonae. Antimicrob Agents Chemother. 2009; 53(4):1367-76. PMC: 2663066. DOI: 10.1128/AAC.01275-08. View

Nikolenko S, Korobeynikov A, Alekseyev M . BayesHammer: Bayesian clustering for error correction in single-cell sequencing. BMC Genomics. 2013; 14 Suppl 1:S7. PMC: 3549815. DOI: 10.1186/1471-2164-14-S1-S7. View

10.

Brode S, Daley C, Marras T . The epidemiologic relationship between tuberculosis and non-tuberculous mycobacterial disease: a systematic review. Int J Tuberc Lung Dis. 2014; 18(11):1370-7. DOI: 10.5588/ijtld.14.0120. View

11.

Rubio M, March F, Garrigo M, Moreno C, Espanol M, Coll P . Inducible and Acquired Clarithromycin Resistance in the Mycobacterium abscessus Complex. PLoS One. 2015; 10(10):e0140166. PMC: 4598034. DOI: 10.1371/journal.pone.0140166. View

12.

Mougari F, Bouziane F, Crockett F, Nessar R, Chau F, Veziris N . Selection of Resistance to Clarithromycin in Mycobacterium abscessus Subspecies. Antimicrob Agents Chemother. 2016; 61(1). PMC: 5192163. DOI: 10.1128/AAC.00943-16. View

13.

Somerville W, Thibert L, Schwartzman K, Behr M . Extraction of Mycobacterium tuberculosis DNA: a question of containment. J Clin Microbiol. 2005; 43(6):2996-7. PMC: 1151963. DOI: 10.1128/JCM.43.6.2996-2997.2005. View

14.

Fan L, Xu J . Advantages and drawbacks of long-term macrolide use in the treatment of non-cystic fibrosis bronchiectasis. J Thorac Dis. 2014; 6(7):867-71. PMC: 4120182. DOI: 10.3978/j.issn.2072-1439.2014.07.24. View

15.

Griffith D, Aksamit T, Brown-Elliott B, Catanzaro A, Daley C, Gordin F . An official ATS/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. Am J Respir Crit Care Med. 2007; 175(4):367-416. DOI: 10.1164/rccm.200604-571ST. View

16.

Nessar R, Reyrat J, Murray A, Gicquel B . Genetic analysis of new 16S rRNA mutations conferring aminoglycoside resistance in Mycobacterium abscessus. J Antimicrob Chemother. 2011; 66(8):1719-24. PMC: 3133489. DOI: 10.1093/jac/dkr209. View

17.

Marras T, Mendelson D, Marchand-Austin A, May K, Jamieson F . Pulmonary nontuberculous mycobacterial disease, Ontario, Canada, 1998-2010. Emerg Infect Dis. 2013; 19(11):1889-91. PMC: 3837646. DOI: 10.3201/eid1911.130737. View

18.

Bastian S, Veziris N, Roux A, Brossier F, Gaillard J, Jarlier V . Assessment of clarithromycin susceptibility in strains belonging to the Mycobacterium abscessus group by erm(41) and rrl sequencing. Antimicrob Agents Chemother. 2010; 55(2):775-81. PMC: 3028756. DOI: 10.1128/AAC.00861-10. View

19.

Maurer F, Ruegger V, Ritter C, Bloemberg G, Bottger E . Acquisition of clarithromycin resistance mutations in the 23S rRNA gene of Mycobacterium abscessus in the presence of inducible erm(41). J Antimicrob Chemother. 2012; 67(11):2606-11. DOI: 10.1093/jac/dks279. View

20.

Xu Z, Ding K, Huang H, Sun T, Ke H, Tong Z . [Questionnaire survey of guideline implementation for community acquired pneumonia with retrospective analysis of the empiric treatment in 490 patients]. Zhonghua Jie He He Hu Xi Za Zhi. 2007; 30(6):442-6. View