Insulin Attenuates TNFα-induced Hemopexin MRNA: An Anti-inflammatory Action of Insulin in Rat H4IIE Hepatoma Cells

Overview

Journal Biochem Biophys Rep

Specialty Biochemistry

Date 2017 Sep 29

PMID 28956007

Citations 4

Authors

J Lee Franklin

William L Bennett

Joseph L Messina

Affiliations

Soon will be listed here.

Abstract

Proinflammatory cytokines, including TNF-α and IL-6, can contribute to insulin resistance. Conversely, insulin has some actions that can be considered anti-inflammatory. Hemopexin is a Class 2 acute phase reactant and control of its transcription is predominantly regulated by IL-6, with TNF-α and IL-1β also inducing hemopexin gene expression. Thus, we asked whether insulin could inhibit the ability of TNF-α to stimulate hemopexin mRNA expression. In cultured rat hepatoma (H4IIE) cells, TNF-α significantly increased hemopexin mRNA accumulation. The TNF-α-induced increase of hemopexin mRNA was dramatically attenuated by insulin, even though TNF-α reduced peak insulin activation of ERK. Thus, even though TNF-α can contribute to insulin resistance, the residual insulin response was still able to counteract TNF-α actions.

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