Antibody Response to Glycan Antigens of Hydatid Cyst Fluid, Laminated Layer and Protoscolex of

Overview

Journal Med J Islam Repub Iran

Specialty General Medicine

Date 2017 Sep 29

PMID 28955662

Citations 2

Authors

Raheleh Rafiei Sefiddashti

Seyedeh Maryam Sharafi

Soltan Ahmad Ebrahimi

Lame Akhlaghi

Ali Moosavi

Abbasali Eskandarian

Alireza Hejrati

Hossein Yousofi Darani

Affiliations

Soon will be listed here.

Abstract

Hydatid disease is characterized by long-term growth of hydatid cysts in the human. The glycan antigens have an important role in the immunology of hydatid cyst. In this study immunological reaction of host sera to different glycan antigens of the cyst, has been investigated. The antibody responses were tested to glycoprotein and glycolipid of the laminated layer (LL), cyst fluid (CF) and protoscolex (PS) antigens of using ELISA and western immunoblotting tests. Thin-layer chromatography and ß-elimination were used for glycan purification. Both hydatid cyst and normal human sera reacted with hydatid cyst fluid, protoscolices, laminated layer, glycoprotein and glycolipid antigens. The most antigen-antibody reaction was related to CF and PS antigens, and LL antigens had the minimal reaction with the sera. Thin layer chromatography (TLC) of the antigens showed presence of many glycan bands in the laminated layer. The parasite may elaborate different glycan antigens in LL to evade host immune response.

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