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Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry (Nano-PALDI MS) with Py-Tag for the Analysis of Small Molecules

Overview
Journal Mass Spectrom (Tokyo)
Date 2017 Sep 27
PMID 28948132
Citations 4
Authors
Yukina Tatsuta
Yukie Tanaka
Akari Ikeda
Shigeru Matsukawa
Hajime Katano
Shu Taira
Affiliations
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Abstract

We compared two ionization methods, matrix assisted laser desorption/ionization (MALDI) and nanoparticle assisted laser desorption/ionization (Nano-PALDI) mass spectrometry (MS), for the analysis of amino acids derivatized with Py-Tag™ that consists pyrylium-based compound. Py-Tag is a useful stable derivatization reagent due to wide mass differences (using C as the sole stable labelling isotope). For Py-Tag labelled lysine, sensitive signals that showed less noise with a ten times higher sensitivity, showed a wider mass difference by Nano-PALDI MS compared to MALDI MS.

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