Aqueous Root Extract of Asparagus Cochinchinensis (Lour.) Merr. Has Antioxidant Activity in D-galactose-induced Aging Mice

Overview

Journal BMC Complement Altern Med

Publisher Biomed Central

Specialty Rehabilitation Medicine

Date 2017 Sep 27

PMID 28946917

Citations 13

Authors

Linghua Lei

Yanhua Chen

Lijun Ou

Yinglong Xu

Xiaoying Yu

Affiliations

Soon will be listed here.

Abstract

Background: Extracts of plants have been considered as sources of natural antioxidant agents. In this study, we aimed to explore the antioxidant capacity of the aqueous root extract of Asparagus cochinchinensis (Lour.) Merr.

Methods: Using vitamin C (Vc) as a positive control, we analyzed the aqueous root extract of A. cochinchinensis free radical scavenging ability in vitro. We also established a mouse aging model using D-galactose and then treated it with aqueous root extract or Vc. The blood cell count and superoxide dismutase (SOD), catalase (CAT), and nitric oxide synthase (NOS) activities as well as malondialdehyde (MDA) and nitric oxide (NO) contents were measured; pathological examination of tissues was performed; and SOD, glutathione peroxidase (GPX), and NOS expression levels in the serum, liver, and brain tissues were investigated.

Results: In vitro, compared with the antioxidant Vc, the aqueous root extract showed similar 1,1-Diphenyl-2-picrylhydrazyl radical and 3-ethylbenzothiazoline-6-sulfonic·scavenging activities and even significantly increased superoxide anion (p < 0.05) and hydroxyl radical (OH) (p < 0.01) scavenging activities. The aqueous extract significantly increased the white blood cell count as well as enhanced SOD, CAT, and NOS activities (p < 0.01) in aging mice. In addition, the aqueous extract increased the NO content (p < 0.05) and reduced the MDA content (p < 0.05).

Conclusions: The aqueous root extract of A. cochinchinensis showed as strong antioxidant ability as Vc and might prevent aging by reducing radicals.

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References

Harman D . Aging: a theory based on free radical and radiation chemistry. J Gerontol. 1956; 11(3):298-300. DOI: 10.1093/geronj/11.3.298. View

Lei L, Ou L, Yu X . The antioxidant effect of Asparagus cochinchinensis (Lour.) Merr. shoot in D-galactose induced mice aging model and in vitro. J Chin Med Assoc. 2016; 79(4):205-11. DOI: 10.1016/j.jcma.2015.06.023. View

Jesko H, Chalimoniuk M, Strosznajder J . Activation of constitutive nitric oxide synthase(s) and absence of inducible isoform in aged rat brain. Neurochem Int. 2002; 42(4):315-22. DOI: 10.1016/s0197-0186(02)00098-0. View

Alvarez-Suarez J, Dekanski D, Ristic S, Radonjic N, Petronijevic N, Giampieri F . Strawberry polyphenols attenuate ethanol-induced gastric lesions in rats by activation of antioxidant enzymes and attenuation of MDA increase. PLoS One. 2011; 6(10):e25878. PMC: 3189224. DOI: 10.1371/journal.pone.0025878. View

Duan S, Tang S, Qin N, Duan H . [Chemical constituents of Phymatopteris hastate and their antioxidant activity]. Zhongguo Zhong Yao Za Zhi. 2012; 37(10):1402-7. View

Fisch K, Bohm V, Wright A, Konig G . Antioxidative meroterpenoids from the brown alga Cystoseira crinita. J Nat Prod. 2003; 66(7):968-75. DOI: 10.1021/np030082f. View

Ambrosio G, Flaherty J, DUILIO C, Tritto I, Santoro G, Elia P . Oxygen radicals generated at reflow induce peroxidation of membrane lipids in reperfused hearts. J Clin Invest. 1991; 87(6):2056-66. PMC: 296962. DOI: 10.1172/JCI115236. View

Zheng H, Zhao J, Liu Y, Zheng Y, Wu J, Hong Z . [Effect of total alkaloids of Rubus alceaefolius on oxidative stress in rats with non-alcoholic fatty liver disease]. Zhongguo Zhong Yao Za Zhi. 2011; 36(17):2383-7. View

Suh H, Chung M, Cho Y, Kim J, Kim D, Han K . Estimated daily intakes of butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and tert-butyl hydroquinone (TBHQ) antioxidants in Korea. Food Addit Contam. 2005; 22(12):1176-88. DOI: 10.1080/02652030500195288. View

10.

Kibbe M, Billiar T, Tzeng E . Inducible nitric oxide synthase and vascular injury. Cardiovasc Res. 2000; 43(3):650-7. DOI: 10.1016/s0008-6363(99)00130-3. View

11.

Salehpour F, Ahmadian N, Rasta S, Farhoudi M, Karimi P, Sadigh-Eteghad S . Transcranial low-level laser therapy improves brain mitochondrial function and cognitive impairment in D-galactose-induced aging mice. Neurobiol Aging. 2017; 58:140-150. DOI: 10.1016/j.neurobiolaging.2017.06.025. View

12.

Reiter R, Tan D, Osuna C, Gitto E . Actions of melatonin in the reduction of oxidative stress. A review. J Biomed Sci. 2000; 7(6):444-58. DOI: 10.1007/BF02253360. View

13.

Law A, ODonnell J, Gauthier S, Quirion R . Neuronal and inducible nitric oxide synthase expressions and activities in the hippocampi and cortices of young adult, aged cognitively unimpaired, and impaired Long-Evans rats. Neuroscience. 2002; 112(2):267-75. DOI: 10.1016/s0306-4522(02)00082-9. View

14.

Dillin A, Gottschling D, Nystrom T . The good and the bad of being connected: the integrons of aging. Curr Opin Cell Biol. 2014; 26:107-12. PMC: 3927154. DOI: 10.1016/j.ceb.2013.12.003. View

15.

Cadet J, Ladenheim B, Baum I, Carlson E, Epstein C . CuZn-superoxide dismutase (CuZnSOD) transgenic mice show resistance to the lethal effects of methylenedioxyamphetamine (MDA) and of methylenedioxymethamphetamine (MDMA). Brain Res. 1994; 655(1-2):259-62. DOI: 10.1016/0006-8993(94)91624-1. View

16.

Melov S, Ravenscroft J, Malik S, Gill M, Walker D, Clayton P . Extension of life-span with superoxide dismutase/catalase mimetics. Science. 2000; 289(5484):1567-9. DOI: 10.1126/science.289.5484.1567. View

17.

Cui X, Zuo P, Zhang Q, Li X, Hu Y, Long J . Chronic systemic D-galactose exposure induces memory loss, neurodegeneration, and oxidative damage in mice: protective effects of R-alpha-lipoic acid. J Neurosci Res. 2006; 83(8):1584-90. DOI: 10.1002/jnr.20845. View

18.

Jalsrai A, Numakawa T, Kunugi H, Dieterich D, Becker A . The neuroprotective effects and possible mechanism of action of a methanol extract from Asparagus cochinchinensis: In vitro and in vivo studies. Neuroscience. 2016; 322:452-63. DOI: 10.1016/j.neuroscience.2016.02.065. View

19.

Lee D, Choo B, Yoon T, Cheon M, Lee H, Lee A . Anti-inflammatory effects of Asparagus cochinchinensis extract in acute and chronic cutaneous inflammation. J Ethnopharmacol. 2008; 121(1):28-34. DOI: 10.1016/j.jep.2008.07.006. View

20.

Sun J, Liu S, Zhang C, Yu L, Bi J, Zhu F . Chemical composition and antioxidant activities of Broussonetia papyrifera fruits. PLoS One. 2012; 7(2):e32021. PMC: 3289642. DOI: 10.1371/journal.pone.0032021. View