Thrombopoietic Stimulating Activity of RhTyrRS (Y341A)

Overview

Journal Sci Rep

Specialty Science

Date 2017 Sep 24

PMID 28939876

Citations 1

Authors

Yu Jinchao

Zhang Yanling

Wang Xu

Zhao Bing

Ye Yuhao

Zhou Weiran

Sun Shaoyang

Ma Liyun

Shi Yun

Zhan Ling

Yu Min

Mo Wei

Affiliations

Soon will be listed here.

Abstract

Tumor radiotherapy induces hematopoietic organ damage and reduces thrombocyte counts. Thrombocytopenia is a common disease. Some studies have shown that tRNA synthetase plays not only catalytic tRNA aminoacylation roles, but also functions similarly to cytokines. Recombinant human tyrosyl-tRNA synthetase with a mutated Y341A (rhTyrRS (Y341A)) promotes megakaryocyte migrate from bone marrow to peripheral blood. It would promote megakaryocytes in the lungs adhering to vascular endothelial cells and resulting in the platelet production. The purpose of this research was to investigate the efficacy of rhTyrRS (Y341A) as a therapy for thrombocytopenia and to explore its mechanism of action. We found platelet number was effectively increased by rhTyrRS (Y341A) via platelet count and reticulated platelets (RPs) flow cytometry. We also demonstrated radiation-induced thrombocytopenia could be prevented by rhTyrRS (Y341A). The results of immunohistochemistry and H&E staining showed the number of pulmonary mature megakaryocytes was significantly increased in rhTyrRS (Y341A) treated groups. In transgenic zebrafish larvae, confocal microscopy results showed rhTyrRS (Y341A) promoted the migration and adhesion of megakaryocytes. These results suggested that rhTyrRS (Y341A) promote megakaryocytes in bone marrow migrating to lungs through blood circulation. rhTyrRS (Y341A) may be an effective medicine which could be used to treat patients suffering from thrombocytopenia.

Citing Articles

Protective Effect and Mechanisms of New Gelatin on Chemotherapy-Induced Hematopoietic Injury Zebrafish Model.

Han L, Kong H, Liu F, Li X, Zhang S, Zhang X Evid Based Complement Alternat Med. 2019; 2019:8918943.

PMID: 31531120 PMC: 6721477. DOI: 10.1155/2019/8918943.

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