Gas Chromatography-Mass Spectrometry for Metabolite Profiling of Japanese Black Cattle Naturally Contaminated with Zearalenone and Sterigmatocystin

Overview

Journal Toxins (Basel)

Publisher MDPI

Specialty Toxicology

Date 2017 Sep 22

PMID 28934162

Citations 6

Authors

Katsuki Toda

Emiko Kokushi

Seiichi Uno

Ayaka Shiiba

Hiroshi Hasunuma

Yasuo Fushimi

Missaka P B Wijayagunawardane

Chunhua Zhang

Osamu Yamato

Masayasu Taniguchi

Johanna Fink-Gremmels

Mitsuhiro Takagi

Affiliations

Soon will be listed here.

Abstract

The objective of this study was to evaluate the metabolic profile of cattle fed with or without zearalenone (ZEN) and sterigmatocystin (STC)-contaminated diets using a gas chromatography-mass spectrometry metabolomics approach. Urinary samples were collected from individual animals ( = 6 per herd) from fattening female Japanese Black (JB) cattle herds (23 months old, 550-600 kg). Herd 1 had persistently high urinary ZEN and STC concentrations due to the presence of contaminated rice straw. Herd 2, the second female JB fattening herd (23 months old, 550-600 kg), received the same dietary feed as Herd 1, with non-contaminated rice straw. Urine samples were collected from Herd 1, two weeks after the contaminated rice straw was replaced with uncontaminated rice straw (Herd 1N). Identified metabolites were subjected to principal component analysis (PCA) and ANOVA. The PCA revealed that the effects on cattle metabolites depended on ZEN and STC concentrations. The contamination of cattle feed with multiple mycotoxins may alter systemic metabolic processes, including metabolites associated with ATP generation, amino acids, glycine-conjugates, organic acids, and purine bases. The results obtained from Herd 1N indicate that a two-week remedy period was not sufficient to improve the levels of urinary metabolites, suggesting that chronic contamination with mycotoxins may have long-term harmful effects on the systemic metabolism of cattle.

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References

Lemmon M, Schlessinger J . Cell signaling by receptor tyrosine kinases. Cell. 2010; 141(7):1117-34. PMC: 2914105. DOI: 10.1016/j.cell.2010.06.011. View

Chorell E, Moritz T, Branth S, Antti H, Svensson M . Predictive metabolomics evaluation of nutrition-modulated metabolic stress responses in human blood serum during the early recovery phase of strenuous physical exercise. J Proteome Res. 2009; 8(6):2966-77. DOI: 10.1021/pr900081q. View

Wan Q, Wu G, He Q, Tang H, Wang Y . The toxicity of acute exposure to T-2 toxin evaluated by the metabonomics technique. Mol Biosyst. 2015; 11(3):882-91. DOI: 10.1039/c4mb00622d. View

Wishart D . Metabolomics: the principles and potential applications to transplantation. Am J Transplant. 2005; 5(12):2814-20. DOI: 10.1111/j.1600-6143.2005.01119.x. View

Moraes E, Ruperez F, Plaza M, Herrero M, Barbas C . Metabolomic assessment with CE-MS of the nutraceutical effect of Cystoseira spp extracts in an animal model. Electrophoresis. 2011; 32(15):2055-62. DOI: 10.1002/elps.201000546. View

Bertram H, Yde C, Zhang X, Kristensen N . Effect of dietary nitrogen content on the urine metabolite profile of dairy cows assessed by nuclear magnetic resonance (NMR)-based metabolomics. J Agric Food Chem. 2011; 59(23):12499-505. DOI: 10.1021/jf204201f. View

Huang X, Shao L, Gong Y, Mao Y, Liu C, Qu H . A metabonomic characterization of CCl4-induced acute liver failure using partial least square regression based on the GC/MS metabolic profiles of plasma in mice. J Chromatogr B Analyt Technol Biomed Life Sci. 2008; 870(2):178-85. DOI: 10.1016/j.jchromb.2008.05.049. View

Fink-Gremmels J . The role of mycotoxins in the health and performance of dairy cows. Vet J. 2008; 176(1):84-92. DOI: 10.1016/j.tvjl.2007.12.034. View

Takagi M, Uno S, Kokushi E, Shiga S, Mukai S, Kuriyagawa T . Measurement of urinary zearalenone concentrations for monitoring natural feed contamination in cattle herds: On-farm trials. J Anim Sci. 2010; 89(1):287-96. DOI: 10.2527/jas.2010-3306. View

10.

Boudra H, Doreau M, Noziere P, Pujos-Guillot E, Morgavi D . Simultaneous analysis of the main markers of nitrogen status in dairy cow's urine using hydrophilic interaction chromatography and tandem mass spectrometry detection. J Chromatogr A. 2012; 1256:169-76. DOI: 10.1016/j.chroma.2012.07.094. View

11.

Bomalaski J, Clark M . Serum uric acid-lowering therapies: where are we heading in management of hyperuricemia and the potential role of uricase. Curr Rheumatol Rep. 2004; 6(3):240-7. DOI: 10.1007/s11926-004-0075-3. View

12.

Osorio M, Moloney A, Brennan L, Monahan F . Authentication of beef production systems using a metabolomic-based approach. Animal. 2012; 6(1):167-72. DOI: 10.1017/S1751731111001418. View

13.

Lasram M, Dhouib I, Annabi A, El Fazaa S, Gharbi N . A review on the molecular mechanisms involved in insulin resistance induced by organophosphorus pesticides. Toxicology. 2014; 322:1-13. DOI: 10.1016/j.tox.2014.04.009. View

14.

Zhang L, Ye Y, An Y, Tian Y, Wang Y, Tang H . Systems responses of rats to aflatoxin B1 exposure revealed with metabonomic changes in multiple biological matrices. J Proteome Res. 2010; 10(2):614-23. DOI: 10.1021/pr100792q. View

15.

Liu G, Yan T, Wang J, Huang Z, Chen X, Jia G . Biological system responses to zearalenone mycotoxin exposure by integrated metabolomic studies. J Agric Food Chem. 2013; 61(46):11212-21. DOI: 10.1021/jf403401v. View

16.

Uno S, Shintoyo A, Kokushi E, Yamamoto M, Nakayama K, Koyama J . Gas chromatography-mass spectrometry for metabolite profiling of Japanese medaka (Oryzias latipes) juveniles exposed to malathion. Environ Sci Pollut Res Int. 2012; 19(7):2595-605. DOI: 10.1007/s11356-012-0834-z. View

17.

Brand B, Hadlich F, Brandt B, Schauer N, Graunke K, Langbein J . Temperament type specific metabolite profiles of the prefrontal cortex and serum in cattle. PLoS One. 2015; 10(4):e0125044. PMC: 4416037. DOI: 10.1371/journal.pone.0125044. View

18.

Vallejo M, Garcia A, Tunon J, Garcia-Martinez D, Angulo S, Martin-Ventura J . Plasma fingerprinting with GC-MS in acute coronary syndrome. Anal Bioanal Chem. 2009; 394(6):1517-24. DOI: 10.1007/s00216-009-2610-6. View

19.

Sharma R, Olson L, STOWE C . Excretion of benzoate in bovine urine after the administration of thiopental. Biochem Pharmacol. 1972; 21(2):181-91. DOI: 10.1016/0006-2952(72)90268-7. View

20.

Fisher S, Heacock A, Agranoff B . Inositol lipids and signal transduction in the nervous system: an update. J Neurochem. 1992; 58(1):18-38. DOI: 10.1111/j.1471-4159.1992.tb09273.x. View