Membrane Fluidity Sensing on the Single Virus Particle Level with Plasmonic Nanoparticle Transducers

Overview

Journal ACS Sens

Publisher American Chemical Society

Specialty Biotechnology

Date 2017 Sep 22

PMID 28933537

Citations 2

Authors

Amin Feizpour

David Stelter

Crystal Wong

Hisashi Akiyama

Suryaram Gummuluru

Tom Keyes

Bjorn M Reinhard

Affiliations

Soon will be listed here.

Abstract

Viral membranes are nanomaterials whose fluidity depends on their composition, in particular, the cholesterol (chol) content. As differences in the membrane composition of individual virus particles can lead to different intracellular fates, biophysical tools capable of sensing the membrane fluidity on the single-virus level are required. In this manuscript, we demonstrate that fluctuations in the polarization of light scattered off gold or silver nanoparticle (NP)-labeled virus-like-particles (VLPs) encode information about the membrane fluidity of individual VLPs. We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level. Chol extraction studies with different methyl-β-cyclodextrin (MβCD) concentrations yielded a gradual decrease in polarization fluctuations as a function of time. The rate of chol extraction for individual VLPs showed a broad spread, presumably due to differences in the membrane composition for the individual VLPs, and this heterogeneity increased with decreasing MβCD concentration.

Citing Articles

Recent Developments in Nanosensors for Imaging Applications in Biological Systems.

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Szalkowski M, Sulowska K, Grzelak J, Niedziolka-Jonsson J, Rozniecka E, Kowalska D Sensors (Basel). 2018; 18(1).

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