Hepatocyte Transplantation: Cell Sheet Technology for Liver Cell Transplantation

Overview

Journal Curr Transplant Rep

Date 2017 Sep 22

PMID 28932649

Citations 23

Authors

Kohei Tatsumi

Teruo Okano

Affiliations

Soon will be listed here.

Abstract

Purpose Of Review: We will review the recent developments of cell sheet technology as a feasible tissue engineering approach. Specifically, we will focus on the technological advancement for engineering functional liver tissue using cell sheet technology, and the associated therapeutic effect of cell sheets for liver diseases, highlighting hemophilia.

Recent Findings: Cell-based therapies using hepatocytes have recently been explored as a new therapeutic modality for patients with many forms of liver disease. We have developed a cell sheet technology, which allows cells to be harvested in a monolithic layer format. We have succeeded in fabricating functional liver tissues in mice by stacking the cell sheets composed of primary hepatocytes. As a curative measure for hemophilia, we have also succeeded in treating hemophilia mice by transplanting of cells sheets composed of genetically modified autologous cells.

Summary: Tissue engineering using cell sheet technology provides the opportunity to create new therapeutic options for patients with various types of liver diseases.

Citing Articles

Development of iPSC-derived FIX-secreting hepatocyte sheet as a novel treatment tool for hemophilia B treatment.

Bayarsaikhan D, Bayarsaikhan G, Lee J, Okano T, Kim K, Lee B Stem Cell Res Ther. 2025; 16(1):88.

PMID: 39988667 PMC: 11849234. DOI: 10.1186/s13287-025-04195-8.

In-Vitro Decidualization With Different Progesterone Concentration: Development of a Hormone-Responsive 3D Endometrial Tissue Using Rat Endometrial Tissues.

Toma C, Kuramoto G, Homma J, Sakaguchi K, Shimizu T Cureus. 2023; 15(11):e49613.

PMID: 38033443 PMC: 10685080. DOI: 10.7759/cureus.49613.

Liver cell therapies: cellular sources and grafting strategies.

Zhang W, Cui Y, Du Y, Yang Y, Fang T, Lu F Front Med. 2023; 17(3):432-457.

PMID: 37402953 DOI: 10.1007/s11684-023-1002-1.

Cell therapy in end-stage liver disease: replace and remodel.

Hu X, Chen L, Wu H, Tang Y, Zheng Q, Wei X Stem Cell Res Ther. 2023; 14(1):141.

PMID: 37231461 PMC: 10210428. DOI: 10.1186/s13287-023-03370-z.

An efficient method for cell sheet bioengineering from rBMSCs on thermo-responsive PCL-PEG-PCL copolymer.

Vaghefi Moghaddam S, Abedi F, Lotfi H, Salehi R, Barzegar A, Baghaban Eslaminejad M J Biol Eng. 2023; 17(1):27.

PMID: 37024910 PMC: 10080813. DOI: 10.1186/s13036-023-00346-8.

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