Isolation and Function of a Clostridium Perfringens Enterotoxin Fragment

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1987 Dec 1

PMID 2890582

Citations 19

Authors

Y Horiguchi

T Akai

G Sakaguchi

Affiliations

Soon will be listed here.

Abstract

A fragment was obtained by treating Clostridium perfringens enterotoxin with 2-nitro-5-thiocyanobenzoic acid, a reagent which specifically cleaves the amino-terminal peptide bond of cysteine residues. The fragment (molecular weight, 15,000) was purified by high-performance liquid chromatography. The fragment had no cytotoxic effect on Vero cells but competitively inhibited enterotoxin-induced 51Cr release. Binding of 125I-labeled fragment to Vero cells was comparable to that of enterotoxin. Moreover, 125I-labeled fragment did not bind to FL cells, which lack receptor for enterotoxin. We conclude that the fragment contains the binding domain of enterotoxin. The amino acid composition of the fragment suggests that it is located on the carboxyl-terminal part of enterotoxin.

Citing Articles

Characterizing the Contributions of Various Clostridium perfringens Enterotoxin Properties to and Permeability Effects.

Shrestha A, Navarro M, Beingesser J, Armien A, Uzal F, McClane B mSphere. 2022; 7(5):e0027622.

PMID: 36069435 PMC: 9599344. DOI: 10.1128/msphere.00276-22.

RIP1, RIP3, and MLKL Contribute to Cell Death Caused by Clostridium perfringens Enterotoxin.

Shrestha A, Mehdizadeh Gohari I, McClane B mBio. 2019; 10(6).

PMID: 31848291 PMC: 6918092. DOI: 10.1128/mBio.02985-19.

Potential Therapeutic Effects of Mepacrine against Enterotoxin in a Mouse Model of Enterotoxemia.

Navarro M, Shrestha A, Freedman J, Beingesser J, McClane B, Uzal F Infect Immun. 2019; 87(4).

PMID: 30642896 PMC: 6434118. DOI: 10.1128/IAI.00670-18.

Enterotoxic Clostridia: Enteric Diseases.

Shrestha A, Uzal F, McClane B Microbiol Spectr. 2018; 6(5).

PMID: 30238869 PMC: 6736584. DOI: 10.1128/microbiolspec.GPP3-0003-2017.

The Potential Therapeutic Agent Mepacrine Protects Caco-2 Cells against Enterotoxin Action.

Freedman J, Hendricks M, McClane B mSphere. 2017; 2(4).

PMID: 28875177 PMC: 5577654. DOI: 10.1128/mSphere.00352-17.

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