Ultra-High Efficient Colony PCR for High Throughput Screening of Bacterial Genes

Overview

Journal Indian J Microbiol

Specialty Microbiology

Date 2017 Sep 15

PMID 28904423

Citations 7

Authors

Mohammad Abu Hena Mostofa Jamal

Satya Priya Sharma

Hea-Jong Chung

Hyeon-Jin Kim

Seong-Tshool Hong

Seungkoo Lee

Affiliations

Soon will be listed here.

Abstract

Current colony PCR methods are not suitable for screening genes encoded in genomic DNA and are limited to host strains. Here, we describe an ultra-high efficient colony PCR method for high throughput screening of bacterial genes embedded in the genomic DNA of any bacterial species. This new technique expands colony PCR method to several hosts as well as offers a rapid, less expensive and reliable bacterial genomic DNA extraction.

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