Efficient Differentiation of Corynebacterium Striatum, Corynebacterium Amycolatum and Corynebacterium Xerosis Clinical Isolates by Multiplex PCR Using Novel Species-specific Primers

Overview

Journal J Microbiol Methods

Specialty Microbiology

Date 2017 Sep 11

PMID 28888870

Citations 6

Authors

Carolina S Santos

Juliana N Ramos

Veronica V Vieira

Carina S Pinheiro

Roberto Meyer

Neuza M Alcantara-Neves

Rommel T Ramos

Artur Silva

Raphael Hirata Jr

Liza Felicori

Carlos Ruiz de Alegria Puig

Jesus Navas

Vasco Azevedo

Ana L Mattos-Guaraldi

Luis G C Pacheco

Affiliations

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Abstract

A multiplex-PCR (mPCR) assay was designed with species-specific primers which generate amplicons of 226bp, 434bp and 106bp for differentiating the species C. striatum, C. amycolatum, and C. xerosis, respectively. mPCR results were 100% in agreement with identifications achieved by 16S rRNA and rpoB gene sequencing and by VITEK-MS.

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