Permanent Draft Genome Sequence of Sp. NRRL B-16219 Reveals the Presence of Canonical Genes, Which Are Highly Homologous to Those Detected in Frankia Dg1 Genome

sp. NRRL B-16219 was directly isolated from a soil sample obtained from the rhizosphere of growing in the USA. Its host plant range includes members of species. Phylogenetically, strain NRRL B-16219 is closely related to " with a 16S rRNA gene similarity of 99.78%. Because of the lack of genetic tools for , our understanding of the bacterial signals involved during the plant infection process and the development of actinorhizal root nodules is very limited. Since the first three genomes were sequenced, additional genome sequences covering more diverse strains have helped provide insight into the depth of the pangenome and attempts to identify bacterial signaling molecules like the rhizobial canonical genes. The genome sequence of sp. strain NRRL B-16219 was generated and assembled into 289 contigs containing 8,032,739 bp with 71.7% GC content. Annotation of the genome identified 6211 protein-coding genes, 561 pseudogenes, 1758 hypothetical proteins and 53 RNA genes including 4 rRNA genes. The NRRL B-16219 draft genome contained genes homologous to the rhizobial common nodulation genes clustered in two areas. The first cluster contains ACIJH genes whereas the second has AB and H genes in the upstream region. Phylogenetic analysis shows that genes are more deeply rooted than their sister groups from rhizobia. PCR-sequencing suggested the widespread occurrence of highly homologous A and B genes in microsymbionts of field collected .