Analysis of Biofilm Production by Clinical Isolates of Pseudomonas Aeruginosa from Patients with Ventilator-associated Pneumonia

Overview

Journal Rev Bras Ter Intensiva

Specialties Critical Care
General Medicine

Date 2017 Sep 7

PMID 28876402

Citations 20

Authors

Jailton Lobo da Costa Lima

Lilian Rodrigues Alves

Jussyegles Niedja Pereira da Paz

Marcelle Aquino Rabelo

Maria Amelia Vieira Maciel

Marcia Maria Camargo de Morais

Affiliations

Soon will be listed here.

Abstract

Objective: To phenotypically evaluate biofilm production by Pseudomonas aeruginosa clinically isolated from patients with ventilator-associated pneumonia.

Methods: Twenty clinical isolates of P. aeruginosa were analyzed, 19 of which were from clinical samples of tracheal aspirate, and one was from a bronchoalveolar lavage sample. The evaluation of the capacity of P. aeruginosa to produce biofilm was verified using two techniques, one qualitative and the other quantitative.

Results: The qualitative technique showed that only 15% of the isolates were considered biofilm producers, while the quantitative technique showed that 75% of the isolates were biofilm producers. The biofilm isolates presented the following susceptibility profile: 53.3% were multidrug-resistant, and 46.7% were multidrug-sensitive.

Conclusion: The quantitative technique was more effective than the qualitative technique for the detection of biofilm production. For the bacterial population analyzed, biofilm production was independent of the susceptibility profile of the bacteria, demonstrating that the therapeutic failure could be related to biofilm production, as it prevented the destruction of the bacteria present in this structure, causing complications of pneumonia associated with mechanical ventilation, including extrapulmonary infections, and making it difficult to treat the infection.

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