High Antigen Processing Machinery Component Expression in Langerhans Cells from Melanoma Patients' Sentinel Lymph Nodes

Overview

Journal Cell Immunol

Publisher Elsevier

Specialties Allergy & Immunology
Cell Biology

Date 2017 Sep 6

PMID 28870403

Citations 6

Authors

Maria Raffaella Romoli

Paola Di Gennaro

Gianni Gerlini

Serena Sestini

Paola Brandani

Soldano Ferrone

Lorenzo Borgognoni

Affiliations

Soon will be listed here.

Abstract

Langerhans cells (LCs) from melanoma patients sentinel lymph nodes (SLN) are poor T cell activators mostly due to an immature immunophenotype. However Antigen Presenting Machinery (APM) role is unknown. We investigated HLA-class I APM components (Delta, LMP-7/10, TAP-1, Calnexin, Tapasin, β2-microglobulin and HLA-A,B,C) in LCs from healthy donors skin and melanoma patients SLN. APM component levels were low in immature epidermal LCs and significantly increased after maturation (p<0.05); their levels were significantly high in SLN LCs (p<0.01). APM component expression correlated with melanoma Breslow's thickness and SLN metastases: HLA-A,B,C level was significantly lower in SLN LCs from thick lesions patients compared with those from thin/intermediate lesions (p<0.05); β2-microglobulin level was significantly higher in positive SLN LCs compared to negative ones (p<0.05). Functionally, SLN LCs did not phagocytose exogenous antigens. These findings extend LCs knowledge indicating that they are not fully impaired by melanoma, contributing to design new LCs-based therapeutic approaches.

Citing Articles

Langerhans Cells in Sentinel Lymph Nodes from Melanoma Patients.

Gerlini G, Susini P, Sestini S, Brandani P, Giannotti V, Borgognoni L Cancers (Basel). 2024; 16(10).

PMID: 38791968 PMC: 11119378. DOI: 10.3390/cancers16101890.

Dendritic Cell Subsets in Melanoma: Pathophysiology, Clinical Prognosis and Therapeutic Exploitation.

Sosa Cuevas E, Saas P, Aspord C Cancers (Basel). 2023; 15(8).

PMID: 37190135 PMC: 10136655. DOI: 10.3390/cancers15082206.

Localized immune surveillance of primary melanoma in the skin deciphered through executable modeling.

Howell R, Davies J, Clarke M, Appios A, Mesquita I, Jayal Y Sci Adv. 2023; 9(15):eadd1992.

PMID: 37043573 PMC: 10096595. DOI: 10.1126/sciadv.add1992.

Langerhans Cells-Revising Their Role in Skin Pathologies.

Neagu M, Constantin C, Jugulete G, Cauni V, Dubrac S, Szollosi A J Pers Med. 2022; 12(12).

PMID: 36556292 PMC: 9782496. DOI: 10.3390/jpm12122072.

Presence of Dendritic Cell Subsets in Sentinel Nodes of Breast Cancer Patients Is Related to Nodal Burden.

Szpor J, Streb J, Glajcar A, Sadowski P, Streb-Smolen A, Jach R Int J Mol Sci. 2022; 23(15).

PMID: 35955602 PMC: 9369399. DOI: 10.3390/ijms23158461.

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