Molecular and Phytochemical Analysis of Wild Type and Olive Cultivars Grown Under Saudi Arabian Environment

Overview

Journal 3 Biotech

Publisher Springer

Specialty Biotechnology

Date 2017 Sep 5

PMID 28868216

Citations 3

Authors

Abdullah A Abood

Abdullah M Al-Ansari

Hussein M Migdadi

Mohammad K Okla

Abdulaziz M Assaeed

Ahmad K Hegazy

Aref M Alshameri

Mohamad Altaf Khan

Affiliations

Soon will be listed here.

Abstract

This study aimed to assess genetic variability at molecular and phytochemical levels among the four most commonly grown olive cultivars and the wild-type olive of Saudi Arabia. Sixty-six and 80 amplicons were generated from 9 random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) primers, each, producing an average of 95.9 and 86.44% polymorphism for the two markers, respectively. The PIC values were 82.2% for the RAPD and 85.4% for the ISSR markers and the discrimination power for both the markers was 11.1%. The UPGMA cluster analysis based on the RAPD and ISSR data resulted in the aggregation of cultivars and wild accession with a good bootstrapping value according to their origin. Furthermore, a total of 199 compounds were identified in the cultivars based on peak area, retention time, and molecular formula using GC-MS analyses of methanolic and ethanolic extracts. These compounds were classified according to their chemical class; most of them were fatty acids, alcoholic compounds, carboxylic acids, aldehydes, heterocyclic compounds, ketones, alkanes, and phenols. Genetic and phytochemical distances were significantly correlated, based on the Mantel test. The Saudi wild accession also had high numbers of fatty acids and their esters, and can be used in breeding programs for generating new genotypes with interesting characters.

Citing Articles

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ISSR-Based Genetic Diversity Assessment of Genus L. (Oleaceae) from Pakistan.

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Genetic Identification of the Wild Form of Olive () Using Allele-Specific Real-Time PCR.

Kyriakopoulou C, Kalogianni D Foods. 2020; 9(4).

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