Multilayered Magnetic Nanobeads for the Delivery of Peptides Molecules Triggered by Intracellular Proteases

Overview

Journal ACS Appl Mater Interfaces

Publisher American Chemical Society

Specialties Biomedical Engineering
Biotechnology

Date 2017 Sep 1

PMID 28858466

Citations 1

Authors

Alessandra Quarta

Marina Rodio

Marco Cassani

Giuseppe Gigli

Teresa Pellegrino

Loretta L Del Mercato

Affiliations

Soon will be listed here.

Abstract

In this work, the versatility of layer-by-layer technology was combined with the magnetic response of iron oxide nanobeads to prepare magnetic mesostructures with a degradable multilayer shell into which a dye quenched ovalbumin conjugate (DQ-OVA) was loaded. The system was specifically designed to prove the protease sensitivity of the hybrid mesoscale system and the easy detection of the ovalbumin released. The uptake of the nanostructures in the breast cancer cells was followed by the effective release of DQ-OVA upon activation via the intracellular proteases degradation of the polymer shells. Monitoring the fluorescence rising due to DQ-OVA digestion and the cellular dye distribution, together with the electron microscopy studying, enabled us to track the shell degradation and the endosomal uptake pathway that resulted in the release of the digested fragments of DQ ovalbumin in the cytosol.

Citing Articles

Protease-Mediated Contrast Enhancement of Multilayered Magneto-Gadolinium Nanostructures for Imaging and Magnetic Hyperthermia.

Avugadda S, Soni N, Rodrigues E, Persano S, Pellegrino T ACS Appl Mater Interfaces. 2024; 16(6):6743-6755.

PMID: 38295315 PMC: 10875642. DOI: 10.1021/acsami.3c13914.

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