Reduced Expression of Paternally Expressed Gene-3 Enhances Somatic Cell Reprogramming Through Mitochondrial Activity Perturbation

Overview

Journal Sci Rep

Specialty Science

Date 2017 Aug 31

PMID 28852087

Citations 8

Authors

Ilda Theka

Francesco Sottile

Francesco Aulicino

Alvaro Castells Garcia

Maria Pia Cosma

Affiliations

Soon will be listed here.

Abstract

Imprinted genes control several cellular and metabolic processes in embryonic and adult tissues. In particular, paternally expressed gene-3 (Peg3) is active in the adult stem cell population and during muscle and neuronal lineage development. Here we have investigated the role of Peg3 in mouse embryonic stem cells (ESCs) and during the process of somatic cell reprogramming towards pluripotency. Our data show that Peg3 knockdown increases expression of pluripotency genes in ESCs and enhances reprogramming efficiency of both mouse embryonic fibroblasts and neural stem cells. Interestingly, we observed that altered activity of Peg3 correlates with major perturbations of mitochondrial gene expression and mitochondrial function, which drive metabolic changes during somatic cell reprogramming. Overall, our study shows that Peg3 is a regulator of pluripotent stem cells and somatic cell reprogramming.

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