A Comprehensive Study of the Interaction Between Peptidoglycan Fragments and the Extracellular Domain of Mycobacterium Tuberculosis Ser/Thr Kinase PknB

Overview

Journal Chembiochem

Specialty Biochemistry

Date 2017 Aug 30

PMID 28851116

Citations 5

Authors

Qianqian Wang

Roberta Marchetti

Sladjana Prisic

Kentaro Ishii

Yohei Arai

Ippei Ohta

Shinsuke Inuki

Susumu Uchiyama

Alba Silipo

Antonio Molinaro

Robert N Husson

Koichi Fukase

Yukari Fujimoto

Affiliations

Soon will be listed here.

Abstract

The Mycobacterium tuberculosis Ser/Thr kinase PknB is implicated in the regulation of bacterial cell growth and cell division. The intracellular kinase function of PknB is thought to be triggered by peptidoglycan (PGN) fragments that are recognized by the extracytoplasmic domain of PknB. The PGN in the cell wall of M. tuberculosis has several unusual modifications, including the presence of N-glycolyl groups (in addition to N-acetyl groups) in the muramic acid residues and amidation of d-Glu in the peptide chains. Using synthetic PGN fragments incorporating these diverse PGN structures, we analyzed their binding characters through biolayer interferometry (BLI), NMR spectroscopy, and native mass spectrometry (nMS) techniques. The results of BLI showed that muropeptides containing 1,6-anhydro-MurNAc and longer glycan chains exhibited higher binding potency and that the fourth amino acid of the peptide stem, d-Ala, was crucial for protein recognition. Saturation transfer difference (STD) NMR spectroscopy indicated the major involvement of the stem peptide region in the PASTA-PGN fragment binding. nMS suggested that the binding stoichiometry was 1:1. The data provide the first molecular basis for the specific interaction of PGN with PknB and firmly establish PGNs as the effective ligands of PknB.

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