» Articles » PMID: 2884122

Stimulation of Soluble Guanylate Cyclase by Endothelium-derived Relaxing Factor from Cultured Endothelial Cells

Overview
Journal Eur J Pharmacol
Specialty Pharmacology
Date 1987 Mar 17
PMID 2884122
Citations 18
Authors
Affiliations
Soon will be listed here.
Abstract

Bovine endothelial cells, grown on microcarrier beads and superfused with a saline solution, were stimulated with thimerosal or bradykinin to release endothelium-derived relaxing factor (EDRF). EDRF activity in the effluent was assayed in endothelium-denuded rabbit aorta. The stimulation of purified soluble guanylate cyclase in test tubes by the EDRF-containing effluent amounted to 90-fold of basal activity and its time course correlated with that of the dilator response of the aorta. After preincubation of endothelial cells with gossypol the EDRF-induced dilator response as well as the stimulation of guanylate cyclase was suppressed.

Citing Articles

Fendiline and calmidazolium enhance the release of endothelium-derived relaxant factor and of prostacyclin from cultured endothelial cells.

Busse R, Luckhoff A, Winter I, Mulsch A, Pohl U Naunyn Schmiedebergs Arch Pharmacol. 1988; 337(1):79-84.

PMID: 3285226 DOI: 10.1007/BF00169481.


Differential role of extra- and intracellular calcium in the release of EDRF and prostacyclin from cultured endothelial cells.

Luckhoff A, Pohl U, Mulsch A, Busse R Br J Pharmacol. 1988; 95(1):189-96.

PMID: 3064851 PMC: 1854120. DOI: 10.1111/j.1476-5381.1988.tb16564.x.


Mechanisms of action of lipoxygenase and cytochrome P-450-mono-oxygenase inhibitors in blocking endothelium-dependent vasodilatation.

Forstermann U, Alheid U, Frolich J, Mulsch A Br J Pharmacol. 1988; 93(3):569-78.

PMID: 2897218 PMC: 1853851. DOI: 10.1111/j.1476-5381.1988.tb10312.x.


Endothelium-derived relaxant factor inhibits platelet activation.

Busse R, Luckhoff A, Bassenge E Naunyn Schmiedebergs Arch Pharmacol. 1987; 336(5):566-71.

PMID: 2830546 DOI: 10.1007/BF00169315.


Endothelium-derived relaxing factor produced and released from artery and vein is nitric oxide.

Ignarro L, Buga G, Wood K, Byrns R, Chaudhuri G Proc Natl Acad Sci U S A. 1987; 84(24):9265-9.

PMID: 2827174 PMC: 299734. DOI: 10.1073/pnas.84.24.9265.