Effects of Endothelial and Mesenchymal Stem Cells on Improving Myocardial Function in a Sheep Animal Model

Overview

Journal J Tehran Heart Cent

Publisher Tehran University of Medical Sciences

Specialty Cardiology & Vascular Diseases

Date 2017 Aug 23

PMID 28828021

Citations 12

Authors

Shahram Rabbani

Masoud Soleimani

Mohammad Sahebjam

Mohammad Imani

Seyed Mahdi Nassiri

Amir Atashi

Morteza Daliri Joupari

Ali Ghiaseddin

Mostafa Latifpour

Seyed Hossein Ahmadi Tafti

Affiliations

Soon will be listed here.

Abstract

Myocardial infarction is the main cause of death worldwide. Angiogenesis, a promising new therapy for the treatment of diffuse coronary artery disease, shows a poor response to conventional revascularization techniques. This study focused on improving myocardial function using endothelial cells (ECs) and mesenchymal stem cells (MSCs) in a sheep animal model. Acute myocardial infarction was induced in 18 sheep (12 treated cases and 6 controls). Autologous MSCs and ECs were injected in the infarcted area and the border zone. Two months after transplantation, echocardiography, electron microscopy, and immunohistochemistry were performed. Echocardiography in both MSC and EC groups revealed a significant improvement in the ejection fraction compared with the control group (p value < 0.05). Vascular density, estimated by antibodies against the von Willebrand factor and smooth muscle actin, increased in both study groups. The pattern of vascularity in the MSC and EC groups was diffused. The electron microscopic evaluation of the infracted areas revealed cardiomyocytes in variable stages of development in the border zone in both EC and MSC groups. Both ECs and MSCs were able to promote angiogenesis and improve cardiac function. Presumably, MSCs differentiate into ECs and cause angiogenesis as it occurs for ECs.

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References

Gnecchi M, Melo L . Bone marrow-derived mesenchymal stem cells: isolation, expansion, characterization, viral transduction, and production of conditioned medium. Methods Mol Biol. 2008; 482:281-94. DOI: 10.1007/978-1-59745-060-7_18. View

Miyahara Y, Nagaya N, Kataoka M, Yanagawa B, Tanaka K, Hao H . Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarction. Nat Med. 2006; 12(4):459-65. DOI: 10.1038/nm1391. View

Shabbir A, Zisa D, Suzuki G, Lee T . Heart failure therapy mediated by the trophic activities of bone marrow mesenchymal stem cells: a noninvasive therapeutic regimen. Am J Physiol Heart Circ Physiol. 2009; 296(6):H1888-97. PMC: 2716100. DOI: 10.1152/ajpheart.00186.2009. View

Martens T, See F, Schuster M, Sondermeijer H, Hefti M, Zannettino A . Mesenchymal lineage precursor cells induce vascular network formation in ischemic myocardium. Nat Clin Pract Cardiovasc Med. 2006; 3 Suppl 1:S18-22. DOI: 10.1038/ncpcardio0404. View

Babu G, Walker J, Yellon D, Hausenloy D . Peri-procedural myocardial injury during percutaneous coronary intervention: an important target for cardioprotection. Eur Heart J. 2010; 32(1):23-31. DOI: 10.1093/eurheartj/ehq393. View

Makkar R, Price M, Lill M, Frantzen M, Takizawa K, Kleisli T . Intramyocardial injection of allogenic bone marrow-derived mesenchymal stem cells without immunosuppression preserves cardiac function in a porcine model of myocardial infarction. J Cardiovasc Pharmacol Ther. 2005; 10(4):225-33. DOI: 10.1177/107424840501000403. View

Gruh I, Beilner J, Blomer U, Schmiedl A, Schmidt-Richter I, Kruse M . No evidence of transdifferentiation of human endothelial progenitor cells into cardiomyocytes after coculture with neonatal rat cardiomyocytes. Circulation. 2006; 113(10):1326-34. DOI: 10.1161/CIRCULATIONAHA.105.559005. View

Ball S, Shuttleworth A, Kielty C . Direct cell contact influences bone marrow mesenchymal stem cell fate. Int J Biochem Cell Biol. 2004; 36(4):714-27. DOI: 10.1016/j.biocel.2003.10.015. View

Tse H, Siu C, Zhu S, Songyan L, Zhang Q, Lai W . Paracrine effects of direct intramyocardial implantation of bone marrow derived cells to enhance neovascularization in chronic ischaemic myocardium. Eur J Heart Fail. 2007; 9(8):747-53. DOI: 10.1016/j.ejheart.2007.03.008. View

10.

Dai W, Hale S, Martin B, Kuang J, Dow J, Wold L . Allogeneic mesenchymal stem cell transplantation in postinfarcted rat myocardium: short- and long-term effects. Circulation. 2005; 112(2):214-23. DOI: 10.1161/CIRCULATIONAHA.104.527937. View

11.

Kim E, Li R, Weisel R, Mickle D, Jia Z, Tomita S . Angiogenesis by endothelial cell transplantation. J Thorac Cardiovasc Surg. 2001; 122(5):963-71. DOI: 10.1067/mtc.2001.117623. View

12.

Krijnen P, Nijmeijer R, Meijer C, Visser C, Hack C, Niessen H . Apoptosis in myocardial ischaemia and infarction. J Clin Pathol. 2002; 55(11):801-11. PMC: 1769793. DOI: 10.1136/jcp.55.11.801. View

13.

Mann D, Bristow M . Mechanisms and models in heart failure: the biomechanical model and beyond. Circulation. 2005; 111(21):2837-49. DOI: 10.1161/CIRCULATIONAHA.104.500546. View

14.

Schuh A, Liehn E, Sasse A, Hristov M, Sobota R, Kelm M . Transplantation of endothelial progenitor cells improves neovascularization and left ventricular function after myocardial infarction in a rat model. Basic Res Cardiol. 2007; 103(1):69-77. DOI: 10.1007/s00395-007-0685-9. View

15.

Fisher S, Zhang H, Doree C, Mathur A, Martin-Rendon E . Stem cell treatment for acute myocardial infarction. Cochrane Database Syst Rev. 2015; (9):CD006536. PMC: 8572033. DOI: 10.1002/14651858.CD006536.pub4. View

16.

Premaratne G, Ma L, Fujita M, Lin X, Bollano E, Fu M . Stromal vascular fraction transplantation as an alternative therapy for ischemic heart failure: anti-inflammatory role. J Cardiothorac Surg. 2011; 6:43. PMC: 3079611. DOI: 10.1186/1749-8090-6-43. View

17.

Tomita S, Li R, Weisel R, Mickle D, Kim E, Sakai T . Autologous transplantation of bone marrow cells improves damaged heart function. Circulation. 1999; 100(19 Suppl):II247-56. DOI: 10.1161/01.cir.100.suppl_2.ii-247. View

18.

Furlani A, Kalil R, Castro I, Canedo-Delgado A, Barra M, Prates P . Effects of therapeutic angiogenesis with plasmid VEGF165 on ventricular function in a canine model of chronic myocardial infarction. Rev Bras Cir Cardiovasc. 2009; 24(2):143-9. DOI: 10.1590/s0102-76382009000200009. View

19.

Joggerst S, Hatzopoulos A . Stem cell therapy for cardiac repair: benefits and barriers. Expert Rev Mol Med. 2009; 11:e20. DOI: 10.1017/S1462399409001124. View

20.

Grauss R, van Tuyn J, Steendijk P, Winter E, Pijnappels D, Hogers B . Forced myocardin expression enhances the therapeutic effect of human mesenchymal stem cells after transplantation in ischemic mouse hearts. Stem Cells. 2008; 26(4):1083-93. DOI: 10.1634/stemcells.2007-0523. View