BLADE-ON-PETIOLE Proteins Act in an E3 Ubiquitin Ligase Complex to Regulate PHYTOCHROME INTERACTING FACTOR 4 Abundance

Overview

Journal Elife

Specialty Biology

Date 2017 Aug 23

PMID 28826468

Citations 64

Authors

Bo Zhang

Mattias Holmlund

Severine Lorrain

Mikael Norberg

Laszlo Bako

Christian Fankhauser

Ove Nilsson

Affiliations

Soon will be listed here.

Abstract

Both light and temperature have dramatic effects on plant development. Phytochrome photoreceptors regulate plant responses to the environment in large part by controlling the abundance of PHYTOCHROME INTERACTING FACTOR (PIF) transcription factors. However, the molecular determinants of this essential signaling mechanism still remain largely unknown. Here, we present evidence that the () genes, which have previously been shown to control leaf and flower development in Arabidopsis, are involved in controlling the abundance of PIF4. Genetic analysis shows that promotes photo-morphogenesis and modulates thermomorphogenesis by suppressing activity, through a reduction in PIF4 protein level. In red-light-grown seedlings PIF4 ubiquitination was reduced in the mutant. Moreover, we found that BOP proteins physically interact with both PIF4 and CULLIN3A and that a CULLIN3-BOP2 complex ubiquitinates PIF4 in vitro. This shows that BOP proteins act as substrate adaptors in a CUL3 E3 ubiquitin ligase complex, targeting PIF4 proteins for ubiquitination and subsequent degradation.

Citing Articles

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