Identification and Cell Type Specificity of the Tyrosine Hydroxylase Gene Promoter

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1987 Mar 11

PMID 2882469

Citations 14

Authors

C A Harrington

E J Lewis

D Krzemien

D M Chikaraishi

Affiliations

Soon will be listed here.

Abstract

Genomic DNA encoding the rat tyrosine hydroxylase (TH) gene was isolated from a lambda phage library using a nick-translated fragment from a cDNA clone for rat TH. We have determined the initiation site for TH RNA synthesis and have sequenced 1100 bases of the primary transcript and 5' flanking region. The 5' end of the transcript is the same in several rat tissues in which TH is expressed as well as in rat pheochromocytoma cells (PC). RNA prepared from PC cells that had been stimulated with dexamethasone also mapped to the same transcription start site. Sequence upstream from the initiation site contains the canonical TATA box, but no apparent CAAT box. When a portion of the 5' flanking region of the TH gene (-773 to + 27) is fused to the chloramphenicol acetyltransferase (CAT) gene, it promotes expression of CAT in pheochromocytoma cells and GH4 cells, but not in two neural tumour lines, RT4-D and B103, nor in several non neural cell lines. This suggests that this region of the TH gene has features that confer tissue-restricted expression on the TH promoter.

Citing Articles

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A novel basal promoter element is required for expression of the rat tyrosine hydroxylase gene.

Patankar S, LAZAROFF M, Yoon S, Chikaraishi D J Neurosci. 1997; 17(11):4076-86.

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