Novel Polymeric Composites Based on Carboxymethyl Chitosan and Poly(acrylic Acid): in Vitro and in Vivo Evaluation

Overview

Journal J Mater Sci Mater Med

Publisher Springer

Specialty Biomedical Engineering

Date 2017 Aug 21

PMID 28823104

Citations 5

Authors

Qurat-Ul-Ain Sharif

Muhammad Sohail

Mahmood Ahmad

Muhammad Usman Minhas

Shahzeb Khan

Samiullah Khan

Mubeen Kousar

Affiliations

Soon will be listed here.

Abstract

The purpose of the study was to develop a novel, efficient, stable, chemically crosslinked polymeric system that have pH responsive behaviour and can effectively release 5-FU in a controlled manner. Furthermore it can target colonic cancer minimizing the side effects of in vivo chemotherapy via 5-FU. Swelling and drug release studies were performed to evaluate its in vitro release behaviour. Hydrogels were also characterized by FTIR, SEM and DSC. In vitro cytocompatibility and cytotoxicity of the hydrogels were determined by MTT assay using HeLa cells. Developed hydrogels were then administered to rabbits orally to evaluate its pharmacokinetic behaviour in vivo. Maximum swelling, drug loading and release were observed at pH 7.4. Similarly maximum absorption was achieved at pH 7.4 in rabbits. It is concluded that CMC-co-poly(AA) have a great potential to be used for controlled drug delivery and colonic targeting for the delivery for various anticancer drugs.

Citing Articles

Mucoadhesive carriers for oral drug delivery.

Kumar R, Islam T, Nurunnabi M J Control Release. 2022; 351:504-559.

PMID: 36116580 PMC: 9960552. DOI: 10.1016/j.jconrel.2022.09.024.

Synthesis and Characterization of Carboxymethyl Chitosan Nanosponges with Cyclodextrin Blends for Drug Solubility Improvement.

Rizvi S, Akhtar N, Usman Minhas M, Mahmood A, Khan K Gels. 2022; 8(1).

PMID: 35049590 PMC: 8775084. DOI: 10.3390/gels8010055.

Targeted Antitumor Mechanism of C-PC/CMC-CD55sp Nanospheres in HeLa Cervical Cancer Cells.

Liu G, Xu X, Jiang L, Ji H, Zhu F, Jin B Front Pharmacol. 2020; 11:906.

PMID: 32636744 PMC: 7319041. DOI: 10.3389/fphar.2020.00906.

Synthesis and evaluation of topical hydrogel membranes; a novel approach to treat skin disorders.

Ahmad S, Usman Minhas M, Ahmad M, Sohail M, Khalid Q, Abdullah O J Mater Sci Mater Med. 2018; 29(12):191.

PMID: 30539389 DOI: 10.1007/s10856-018-6191-9.

Increasing the stability of Lumbricus terrestris erythrocruorin via poly(acrylic acid) conjugation.

Spivack K, Tucker M, Zimmerman D, Nicholas M, Abdulmalik O, Comolli N Artif Cells Nanomed Biotechnol. 2018; 46(sup2):1137-1144.

PMID: 29916733 PMC: 6476418. DOI: 10.1080/21691401.2018.1480491.

References

Paharia A, Yadav A, Rai G, Jain S, Pancholi S, Agrawal G . Eudragit-coated pectin microspheres of 5-fluorouracil for colon targeting. AAPS PharmSciTech. 2007; 8(1):12. PMC: 2750447. DOI: 10.1208/pt0801012. View

Yadav H, Shivakumar H . In Vitro and In Vivo Evaluation of pH-Sensitive Hydrogels of Carboxymethyl Chitosan for Intestinal Delivery of Theophylline. ISRN Pharm. 2012; 2012:763127. PMC: 3395187. DOI: 10.5402/2012/763127. View

Park J, Saravanakumar G, Kim K, Kwon I . Targeted delivery of low molecular drugs using chitosan and its derivatives. Adv Drug Deliv Rev. 2009; 62(1):28-41. DOI: 10.1016/j.addr.2009.10.003. View

Ranjha N, Ayub G, Naseem S, Ansari M . Preparation and characterization of hybrid pH-sensitive hydrogels of chitosan-co-acrylic acid for controlled release of verapamil. J Mater Sci Mater Med. 2010; 21(10):2805-16. DOI: 10.1007/s10856-010-4134-1. View

Simmonds P . Palliative chemotherapy for advanced colorectal cancer: systematic review and meta-analysis. Colorectal Cancer Collaborative Group. BMJ. 2000; 321(7260):531-5. PMC: 27466. DOI: 10.1136/bmj.321.7260.531. View

Gao X, Cao Y, Song X, Zhang Z, Zhuang X, He C . Biodegradable, pH-responsive carboxymethyl cellulose/poly(acrylic acid) hydrogels for oral insulin delivery. Macromol Biosci. 2013; 14(4):565-75. DOI: 10.1002/mabi.201300384. View

Watanabe T, Itabashi M, Shimada Y, Tanaka S, Ito Y, Ajioka Y . Japanese Society for Cancer of the Colon and Rectum (JSCCR) guidelines 2010 for the treatment of colorectal cancer. Int J Clin Oncol. 2011; 17(1):1-29. DOI: 10.1007/s10147-011-0315-2. View

Yin L, Fei L, Cui F, Tang C, Yin C . Superporous hydrogels containing poly(acrylic acid-co-acrylamide)/O-carboxymethyl chitosan interpenetrating polymer networks. Biomaterials. 2006; 28(6):1258-66. DOI: 10.1016/j.biomaterials.2006.11.008. View

Sohail M, Ahmad M, Usman Minhas M, Ali L, Khalid I, Rashid H . Controlled delivery of valsartan by cross-linked polymeric matrices: Synthesis, in vitro and in vivo evaluation. Int J Pharm. 2015; 487(1-2):110-9. DOI: 10.1016/j.ijpharm.2015.04.013. View

10.

Farag R, Mohamed R . Synthesis and characterization of carboxymethyl chitosan nanogels for swelling studies and antimicrobial activity. Molecules. 2012; 18(1):190-203. PMC: 6269908. DOI: 10.3390/molecules18010190. View

11.

Jiang S, Liu S, Feng W . PVA hydrogel properties for biomedical application. J Mech Behav Biomed Mater. 2011; 4(7):1228-33. DOI: 10.1016/j.jmbbm.2011.04.005. View

12.

Chourasia M, Jain S . Pharmaceutical approaches to colon targeted drug delivery systems. J Pharm Pharm Sci. 2003; 6(1):33-66. View

13.

Usman Minhas M, Ahmad M, Ali L, Sohail M . Synthesis of chemically cross-linked polyvinyl alcohol-co-poly (methacrylic acid) hydrogels by copolymerization; a potential graft-polymeric carrier for oral delivery of 5-fluorouracil. Daru. 2013; 21(1):44. PMC: 3704659. DOI: 10.1186/2008-2231-21-44. View

14.

Ullah F, Othman M, Javed F, Ahmad Z, Akil H . Classification, processing and application of hydrogels: A review. Mater Sci Eng C Mater Biol Appl. 2015; 57:414-33. DOI: 10.1016/j.msec.2015.07.053. View

15.

Van Tomme S, Storm G, Hennink W . In situ gelling hydrogels for pharmaceutical and biomedical applications. Int J Pharm. 2008; 355(1-2):1-18. DOI: 10.1016/j.ijpharm.2008.01.057. View