Self-Assembly DNA Polyplex Vaccine Inside Dissolving Microneedles for High-Potency Intradermal Vaccination

Overview

Journal Theranostics

Date 2017 Aug 19

PMID 28819449

Citations 17

Authors

Jing-Fong Liao

Jin-Ching Lee

Chun-Kuang Lin

Kuo-Chen Wei

Pin-Yuan Chen

Hung-Wei Yang

Affiliations

Soon will be listed here.

Abstract

The strong immunogenicity induction is the powerful weapon to prevent the virus infections. This study demonstrated that one-step synthesis of DNA polyplex vaccine in microneedle (MN) patches can induce high immunogenicity through intradermal vaccination and increase the vaccine stability for storage outside the cold chain. More negative charged DNA vaccine was entrapped into the needle region of MNs followed by DNA polyplex formation with branched polyethylenimine (bPEI) pre-coated in the cavities of polydimethylsiloxane (PDMS) molds that can deliver more DNA vaccine to immune-cell rich epidermis with high transfection efficiency. Our data in this study support the safety and immunogenicity of the MN-based vaccine; the MN patch delivery system induced an immune response 3.5-fold as strong as seen with conventional intramuscular administration; the DNA polyplex formulation provided excellent vaccine stability at high temperature (could be stored at 45ºC for at least 4 months); the DNA vaccine is expected to be manufactured at low cost and not generate sharps waste. We think this study is significant to public health because there is a pressing need for an effective vaccination in developing countries.

Citing Articles

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References

Yang H, Ye L, Guo X, Yang C, Compans R, Prausnitz M . Ebola Vaccination Using a DNA Vaccine Coated on PLGA-PLL/γPGA Nanoparticles Administered Using a Microneedle Patch. Adv Healthc Mater. 2017; 6(1). DOI: 10.1002/adhm.201600750. View

Al-Deen F, Ma C, Xiang S, Selomulya C, Plebanski M, Coppel R . On the efficacy of malaria DNA vaccination with magnetic gene vectors. J Control Release. 2013; 168(1):10-7. DOI: 10.1016/j.jconrel.2013.02.030. View

Kim N, Lee M, Kim K, Lee J, Lee K, Park J . Polyplex-releasing microneedles for enhanced cutaneous delivery of DNA vaccine. J Control Release. 2014; 179:11-7. DOI: 10.1016/j.jconrel.2014.01.016. View

Kim Y, Song J, Lipatov A, Choi S, Lee J, Donis R . Increased immunogenicity of avian influenza DNA vaccine delivered to the skin using a microneedle patch. Eur J Pharm Biopharm. 2012; 81(2):239-47. PMC: 3362687. DOI: 10.1016/j.ejpb.2012.03.010. View

Liu M . DNA vaccines: an historical perspective and view to the future. Immunol Rev. 2011; 239(1):62-84. DOI: 10.1111/j.1600-065X.2010.00980.x. View

Kim Y, Yoo D, Compans R, Kang S, Prausnitz M . Cross-protection by co-immunization with influenza hemagglutinin DNA and inactivated virus vaccine using coated microneedles. J Control Release. 2013; 172(2):579-88. PMC: 3815987. DOI: 10.1016/j.jconrel.2013.04.016. View

Bediz B, Korkmaz E, Khilwani R, Donahue C, Erdos G, Falo Jr L . Dissolvable microneedle arrays for intradermal delivery of biologics: fabrication and application. Pharm Res. 2013; 31(1):117-35. PMC: 3898465. DOI: 10.1007/s11095-013-1137-x. View

Donnelly R, Douroumis D . Microneedles for drug and vaccine delivery and patient monitoring. Drug Deliv Transl Res. 2015; 5(4):311-2. DOI: 10.1007/s13346-015-0250-2. View

Yan G, Arelly N, Farhan N, Lobo S, Li H . Enhancing DNA delivery into the skin with a motorized microneedle device. Eur J Pharm Sci. 2013; 52:215-22. DOI: 10.1016/j.ejps.2013.11.015. View

10.

Wang Q, Zhu D, Liu X, Chen B, Guo X . Microneedles with Controlled Bubble Sizes and Drug Distributions for Efficient Transdermal Drug Delivery. Sci Rep. 2016; 6:38755. PMC: 5144082. DOI: 10.1038/srep38755. View

11.

Kumar A, Wonganan P, Sandoval M, Li X, Zhu S, Cui Z . Microneedle-mediated transcutaneous immunization with plasmid DNA coated on cationic PLGA nanoparticles. J Control Release. 2012; 163(2):230-9. PMC: 3478475. DOI: 10.1016/j.jconrel.2012.08.011. View

12.

Ura T, Okuda K, Shimada M . Developments in Viral Vector-Based Vaccines. Vaccines (Basel). 2015; 2(3):624-41. PMC: 4494222. DOI: 10.3390/vaccines2030624. View

13.

DeMuth P, Su X, Samuel R, Hammond P, Irvine D . Nano-layered microneedles for transcutaneous delivery of polymer nanoparticles and plasmid DNA. Adv Mater. 2010; 22(43):4851-6. PMC: 3030257. DOI: 10.1002/adma.201001525. View

14.

Jones S, Evans K, McElwaine-Johnn H, Sharpe M, Oxford J, Lambkin-Williams R . DNA vaccination protects against an influenza challenge in a double-blind randomised placebo-controlled phase 1b clinical trial. Vaccine. 2009; 27(18):2506-12. DOI: 10.1016/j.vaccine.2009.02.061. View

15.

Koutsonanos D, Compans R, Skountzou I . Targeting the skin for microneedle delivery of influenza vaccine. Adv Exp Med Biol. 2013; 785:121-32. PMC: 6525635. DOI: 10.1007/978-1-4614-6217-0_13. View

16.

Kim J, Han M, Kim Y, Shin S, Nam S, Park J . Tip-loaded dissolving microneedles for transdermal delivery of donepezil hydrochloride for treatment of Alzheimer's disease. Eur J Pharm Biopharm. 2016; 105:148-55. DOI: 10.1016/j.ejpb.2016.06.006. View

17.

Choi C, Lee K, Youn Y, Jang E, Kim W, Min B . Spatially discrete thermal drawing of biodegradable microneedles for vascular drug delivery. Eur J Pharm Biopharm. 2012; 83(2):224-33. DOI: 10.1016/j.ejpb.2012.10.020. View

18.

Chen D, Payne L . Targeting epidermal Langerhans cells by epidermal powder immunization. Cell Res. 2002; 12(2):97-104. DOI: 10.1038/sj.cr.7290115. View

19.

Chen M, Ling M, Lai K, Pramudityo E . Chitosan microneedle patches for sustained transdermal delivery of macromolecules. Biomacromolecules. 2012; 13(12):4022-31. DOI: 10.1021/bm301293d. View

20.

Endmann A, Klunder K, Kapp K, Riede O, Oswald D, Talman E . Cationic lipid-formulated DNA vaccine against hepatitis B virus: immunogenicity of MIDGE-Th1 vectors encoding small and large surface antigen in comparison to a licensed protein vaccine. PLoS One. 2014; 9(7):e101715. PMC: 4081723. DOI: 10.1371/journal.pone.0101715. View