From Single-molecule Spectroscopy to Super-resolution Imaging of the Neuron: a Review

Overview

Journal Methods Appl Fluoresc

Publisher IOP Publishing

Specialties Biochemistry
Chemistry

Date 2017 Aug 16

PMID 28809165

Citations 6

Authors

Romain F Laine

Gabriele S Kaminski Schierle

Sebastian van de Linde

Clemens F Kaminski

Affiliations

Soon will be listed here.

Abstract

For more than 20 years, single-molecule spectroscopy has been providing invaluable insights into nature at the molecular level. The field has received a powerful boost with the development of the technique into super-resolution imaging methods, ca. 10 years ago, which overcome the limitations imposed by optical diffraction. Today, single molecule super-resolution imaging is routinely used in the study of macromolecular function and structure in the cell. Concomitantly, computational methods have been developed that provide information on numbers and positions of molecules at the nanometer-scale. In this overview, we outline the technical developments that have led to the emergence of localization microscopy techniques from single-molecule spectroscopy. We then provide a comprehensive review on the application of the technique in the field of neuroscience research.

Citing Articles

Characterization of individual spores of two biological insecticides, Bacillus thuringiensis and Lysinibacillus sphaericus, in response to glutaraldehyde using single-cell optical approaches.

Chen H, Wang X, Li C, Xu X, Wang G Arch Microbiol. 2024; 206(5):227.

PMID: 38642141 DOI: 10.1007/s00203-024-03941-5.

Imaging VIPER-labeled Cellular Proteins by Correlative Light and Electron Microscopy.

Doh J, Chang Y, Enns C, Lopez C, Beatty K Bio Protoc. 2021; 9(21):e3414.

PMID: 33654913 PMC: 7853974. DOI: 10.21769/BioProtoc.3414.

A Review of Super-Resolution Single-Molecule Localization Microscopy Cluster Analysis and Quantification Methods.

Khater I, Nabi I, Hamarneh G Patterns (N Y). 2020; 1(3):100038.

PMID: 33205106 PMC: 7660399. DOI: 10.1016/j.patter.2020.100038.

Fluorescence anisotropy imaging in drug discovery.

Vinegoni C, Fumene Feruglio P, Gryczynski I, Mazitschek R, Weissleder R Adv Drug Deliv Rev. 2018; 151-152:262-288.

PMID: 29410158 PMC: 6072632. DOI: 10.1016/j.addr.2018.01.019.

Fluorescence Self-Quenching from Reporter Dyes Informs on the Structural Properties of Amyloid Clusters Formed in Vitro and in Cells.

Chen W, Young L, Lu M, Zaccone A, Strohl F, Yu N Nano Lett. 2017; 17(1):143-149.

PMID: 28073262 PMC: 5338000. DOI: 10.1021/acs.nanolett.6b03686.

References

Ando R, Hama H, Yamamoto-Hino M, Mizuno H, Miyawaki A . An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc Natl Acad Sci U S A. 2002; 99(20):12651-6. PMC: 130515. DOI: 10.1073/pnas.202320599. View

van de Linde S, Heilemann M, Sauer M . Live-cell super-resolution imaging with synthetic fluorophores. Annu Rev Phys Chem. 2012; 63:519-40. DOI: 10.1146/annurev-physchem-032811-112012. View

Ram S, Prabhat P, Chao J, Ward E, Ober R . High accuracy 3D quantum dot tracking with multifocal plane microscopy for the study of fast intracellular dynamics in live cells. Biophys J. 2008; 95(12):6025-43. PMC: 2599831. DOI: 10.1529/biophysj.108.140392. View

Kolb H, Finn M, Sharpless K . Click Chemistry: Diverse Chemical Function from a Few Good Reactions. Angew Chem Int Ed Engl. 2001; 40(11):2004-2021. DOI: 10.1002/1521-3773(20010601)40:11<2004::AID-ANIE2004>3.0.CO;2-5. View

Smith C, Joseph N, Rieger B, Lidke K . Fast, single-molecule localization that achieves theoretically minimum uncertainty. Nat Methods. 2010; 7(5):373-5. PMC: 2862147. DOI: 10.1038/nmeth.1449. View

van de Linde S, Krstic I, Prisner T, Doose S, Heilemann M, Sauer M . Photoinduced formation of reversible dye radicals and their impact on super-resolution imaging. Photochem Photobiol Sci. 2010; 10(4):499-506. DOI: 10.1039/c0pp00317d. View

Li D, Shao L, Chen B, Zhang X, Zhang M, Moses B . ADVANCED IMAGING. Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics. Science. 2015; 349(6251):aab3500. PMC: 4659358. DOI: 10.1126/science.aab3500. View

Weiss S . Fluorescence spectroscopy of single biomolecules. Science. 1999; 283(5408):1676-83. DOI: 10.1126/science.283.5408.1676. View

Tinnefeld P, Sauer M . Branching out of single-molecule fluorescence spectroscopy: challenges for chemistry and influence on biology. Angew Chem Int Ed Engl. 2005; 44(18):2642-2671. DOI: 10.1002/anie.200300647. View

10.

Fritschi S, Langer F, Kaeser S, Maia L, Portelius E, Pinotsi D . Highly potent soluble amyloid-β seeds in human Alzheimer brain but not cerebrospinal fluid. Brain. 2014; 137(Pt 11):2909-2915. PMC: 5367518. DOI: 10.1093/brain/awu255. View

11.

Shim S, Xia C, Zhong G, Babcock H, Vaughan J, Huang B . Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes. Proc Natl Acad Sci U S A. 2012; 109(35):13978-83. PMC: 3435176. DOI: 10.1073/pnas.1201882109. View

12.

Deschout H, Zanacchi F, Mlodzianoski M, Diaspro A, Bewersdorf J, Hess S . Precisely and accurately localizing single emitters in fluorescence microscopy. Nat Methods. 2014; 11(3):253-66. DOI: 10.1038/nmeth.2843. View

13.

Lacoste T, Michalet X, Pinaud F, Chemla D, Alivisatos A, Weiss S . Ultrahigh-resolution multicolor colocalization of single fluorescent probes. Proc Natl Acad Sci U S A. 2000; 97(17):9461-6. PMC: 16886. DOI: 10.1073/pnas.170286097. View

14.

Wolter S, Schuttpelz M, Tscherepanow M, VAN DE Linde S, Heilemann M, Sauer M . Real-time computation of subdiffraction-resolution fluorescence images. J Microsc. 2010; 237(1):12-22. DOI: 10.1111/j.1365-2818.2009.03287.x. View

15.

Durisic N, Cuervo L, Lakadamyali M . Quantitative super-resolution microscopy: pitfalls and strategies for image analysis. Curr Opin Chem Biol. 2014; 20:22-8. DOI: 10.1016/j.cbpa.2014.04.005. View

16.

Choi U, Strop P, Vrljic M, Chu S, Brunger A, Weninger K . Single-molecule FRET-derived model of the synaptotagmin 1-SNARE fusion complex. Nat Struct Mol Biol. 2010; 17(3):318-24. PMC: 2922927. DOI: 10.1038/nsmb.1763. View

17.

Heilemann M, Herten D, Heintzmann R, Cremer C, Muller C, Tinnefeld P . High-resolution colocalization of single dye molecules by fluorescence lifetime imaging microscopy. Anal Chem. 2002; 74(14):3511-7. DOI: 10.1021/ac025576g. View

18.

Mortensen K, Churchman L, Spudich J, Flyvbjerg H . Optimized localization analysis for single-molecule tracking and super-resolution microscopy. Nat Methods. 2010; 7(5):377-81. PMC: 3127582. DOI: 10.1038/nmeth.1447. View

19.

Moerner , Kador . Optical detection and spectroscopy of single molecules in a solid. Phys Rev Lett. 1989; 62(21):2535-2538. DOI: 10.1103/PhysRevLett.62.2535. View

20.

Juette M, Gould T, Lessard M, Mlodzianoski M, Nagpure B, Bennett B . Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples. Nat Methods. 2008; 5(6):527-9. DOI: 10.1038/nmeth.1211. View