Spatial Organization and Molecular Interactions of the Schizosaccharomyces Pombe Ccq1-Tpz1-Poz1 Shelterin Complex

Overview

Journal J Mol Biol

Publisher Elsevier

Specialties Microbiology
Molecular Biology

Date 2017 Aug 16

PMID 28807855

Citations 6

Authors

Harry Scott

Jin-Kwang Kim

Clinton Yu

Lan Huang

Feng Qiao

Derek J Taylor

Affiliations

Soon will be listed here.

Abstract

The shelterin complex is a macromolecular assembly of proteins that binds to and protects telomeric DNA, which composes the ends of all linear chromosomes. Shelterin proteins prevent chromosome ends from fusing together and from eliciting erroneous induction of DNA damage response pathways. In addition, shelterin proteins play key roles in regulating the recruitment and activation of telomerase, an enzyme that extends telomeric DNA. In fission yeast, Schizosaccharomyces pombe, interactions between the shelterin proteins Ccq1, Tpz1, and Poz1 are important for regulating telomerase-mediated telomere synthesis and thus telomere length homeostasis. Here, we used electron microscopy combined with genetic labeling to define the three-dimensional arrangement of the S. pombe Ccq1-Tpz1-Poz1 (CTP) complex. Crosslinking mass spectrometry was used to identify individual residues that are in proximity to the protein-protein interfaces of the assembled CTP complex. Together, our data provide a first glimpse into the architectural design of the CTP complex and reveals unique interactions that are important in maintaining the S. pombe telomere in a non-extendible state.

Citing Articles

Structural insights into Pot1-ssDNA, Pot1-Tpz1 and Tpz1-Ccq1 Interactions within fission yeast shelterin complex.

Sun H, Wu Z, Zhou Y, Lu Y, Lu H, Chen H PLoS Genet. 2022; 18(7):e1010308.

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Casein kinase 2 regulates telomere protein complex formation through Rap1 phosphorylation.

Inoue H, Horiguchi M, Ono K, Kanoh J Nucleic Acids Res. 2019; 47(13):6871-6884.

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Development of a Novel Sulfoxide-Containing MS-Cleavable Homobifunctional Cysteine-Reactive Cross-Linker for Studying Protein-Protein Interactions.

Gutierrez C, Block S, Yu C, Soohoo S, Huszagh A, Rychnovsky S Anal Chem. 2018; 90(12):7600-7607.

PMID: 29792801 PMC: 6037416. DOI: 10.1021/acs.analchem.8b01287.

Cross-Linking Mass Spectrometry: An Emerging Technology for Interactomics and Structural Biology.

Yu C, Huang L Anal Chem. 2017; 90(1):144-165.

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Structure of the fission yeast S. pombe telomeric Tpz1-Poz1-Rap1 complex.

Xue J, Chen H, Wu J, Takeuchi M, Inoue H, Liu Y Cell Res. 2017; 27(12):1503-1520.

PMID: 29160296 PMC: 5717408. DOI: 10.1038/cr.2017.145.

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