Correlation and Comparison of Cortical and Hippocampal Neural Progenitor Morphology and Differentiation Through the Use of Micro- and Nano-Topographies

Overview

Journal J Funct Biomater

Specialty Biomedical Engineering

Date 2017 Aug 15

PMID 28805664

Citations 7

Authors

Sharvari Sathe

Xiang Quan Chan

Jing Jin

Erik Bernitt

Hans-Gunther Dobereiner

Evelyn K F Yim

Affiliations

Soon will be listed here.

Abstract

Neuronal morphology and differentiation have been extensively studied on topography. The differentiation potential of neural progenitors has been shown to be influenced by brain region, developmental stage, and time in culture. However, the neurogenecity and morphology of different neural progenitors in response to topography have not been quantitatively compared. In this study, the correlation between the morphology and differentiation of hippocampal and cortical neural progenitor cells was explored. The morphology of differentiated neural progenitors was quantified on an array of topographies. In spite of topographical contact guidance, cell morphology was observed to be under the influence of regional priming, even after differentiation. This influence of regional priming was further reflected in the correlations between the morphological properties and the differentiation efficiency of the cells. For example, neuronal differentiation efficiency of cortical neural progenitors showed a negative correlation with the number of neurites per neuron, but hippocampal neural progenitors showed a positive correlation. Correlations of morphological parameters and differentiation were further enhanced on gratings, which are known to promote neuronal differentiation. Thus, the neurogenecity and morphology of neural progenitors is highly responsive to certain topographies and is committed early on in development.

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References

Xu C, Prince J . Snakes, shapes, and gradient vector flow. IEEE Trans Image Process. 2008; 7(3):359-69. DOI: 10.1109/83.661186. View

Sonam S, Sathe S, Yim E, Sheetz M, Lim C . Cell contractility arising from topography and shear flow determines human mesenchymal stem cell fate. Sci Rep. 2016; 6:20415. PMC: 4754642. DOI: 10.1038/srep20415. View

Tan K, Tann J, Sathe S, Goh S, Ma D, Goh E . Enhanced differentiation of neural progenitor cells into neurons of the mesencephalic dopaminergic subtype on topographical patterns. Biomaterials. 2015; 43:32-43. DOI: 10.1016/j.biomaterials.2014.11.036. View

Florio M, Huttner W . Neural progenitors, neurogenesis and the evolution of the neocortex. Development. 2014; 141(11):2182-94. DOI: 10.1242/dev.090571. View

Li W, Tang Q, Jadhav A, Narang A, Qian W, Shi P . Large-scale topographical screen for investigation of physical neural-guidance cues. Sci Rep. 2015; 5:8644. PMC: 4345323. DOI: 10.1038/srep08644. View

Chua J, Chng C, Moe A, Tann J, Goh E, Chiam K . Extending neurites sense the depth of the underlying topography during neuronal differentiation and contact guidance. Biomaterials. 2014; 35(27):7750-61. DOI: 10.1016/j.biomaterials.2014.06.008. View

Rajkowska G, Miguel-Hidalgo J, Wei J, Dilley G, Pittman S, Meltzer H . Morphometric evidence for neuronal and glial prefrontal cell pathology in major depression. Biol Psychiatry. 1999; 45(9):1085-98. DOI: 10.1016/s0006-3223(99)00041-4. View

Seaberg R, Smukler S, van der Kooy D . Intrinsic differences distinguish transiently neurogenic progenitors from neural stem cells in the early postnatal brain. Dev Biol. 2005; 278(1):71-85. DOI: 10.1016/j.ydbio.2004.10.017. View

Rabinowicz T, Petetot J, Khoury J, de Courten-Myers G . Neocortical maturation during adolescence: change in neuronal soma dimension. Brain Cogn. 2008; 69(2):328-36. DOI: 10.1016/j.bandc.2008.08.005. View

10.

Teo B, Ankam S, Chan L, Yim E . Nanotopography/mechanical induction of stem-cell differentiation. Methods Cell Biol. 2010; 98:241-94. DOI: 10.1016/S0091-679X(10)98011-4. View

11.

Bushong E, Martone M, Ellisman M . Maturation of astrocyte morphology and the establishment of astrocyte domains during postnatal hippocampal development. Int J Dev Neurosci. 2004; 22(2):73-86. DOI: 10.1016/j.ijdevneu.2003.12.008. View

12.

Dowell-Mesfin N, Abdul-Karim M, Turner A, Schanz S, Craighead H, Roysam B . Topographically modified surfaces affect orientation and growth of hippocampal neurons. J Neural Eng. 2005; 1(2):78-90. DOI: 10.1088/1741-2560/1/2/003. View

13.

Macleod D, Dowman J, Hammond R, Leete T, Inoue K, Abeliovich A . The familial Parkinsonism gene LRRK2 regulates neurite process morphology. Neuron. 2006; 52(4):587-93. DOI: 10.1016/j.neuron.2006.10.008. View

14.

Su C, Yoon S, Marcy G, Chin E, Augustine G, Goh E . An optogenetic approach for assessing formation of neuronal connections in a co-culture system. J Vis Exp. 2015; (96):e52408. PMC: 4354644. DOI: 10.3791/52408. View

15.

Radio N, Breier J, Shafer T, Mundy W . Assessment of chemical effects on neurite outgrowth in PC12 cells using high content screening. Toxicol Sci. 2008; 105(1):106-18. DOI: 10.1093/toxsci/kfn114. View

16.

Ankam S, Suryana M, Chan L, Moe A, Teo B, Law J . Substrate topography and size determine the fate of human embryonic stem cells to neuronal or glial lineage. Acta Biomater. 2012; 9(1):4535-45. DOI: 10.1016/j.actbio.2012.08.018. View

17.

Weber S, Fernandez-Cachon M, M Nascimento J, Knauer S, Offermann B, Murphy R . Label-free detection of neuronal differentiation in cell populations using high-throughput live-cell imaging of PC12 cells. PLoS One. 2013; 8(2):e56690. PMC: 3579923. DOI: 10.1371/journal.pone.0056690. View

18.

Arimura N, Kaibuchi K . Neuronal polarity: from extracellular signals to intracellular mechanisms. Nat Rev Neurosci. 2007; 8(3):194-205. DOI: 10.1038/nrn2056. View

19.

Baj G, Patrizio A, Montalbano A, Sciancalepore M, Tongiorgi E . Developmental and maintenance defects in Rett syndrome neurons identified by a new mouse staging system in vitro. Front Cell Neurosci. 2014; 8:18. PMC: 3914021. DOI: 10.3389/fncel.2014.00018. View

20.

Gal J, Morozov Y, Ayoub A, Chatterjee M, Rakic P, Haydar T . Molecular and morphological heterogeneity of neural precursors in the mouse neocortical proliferative zones. J Neurosci. 2006; 26(3):1045-56. PMC: 3249619. DOI: 10.1523/JNEUROSCI.4499-05.2006. View