The Stress-induced SCP/HLIP Family of Small Light-harvesting-like Proteins (ScpABCDE) Protects Photosystem II from Photoinhibitory Damages in the Cyanobacterium Synechocystis Sp. PCC 6803

Overview

Journal Photosynth Res

Publisher Springer

Date 2017 Aug 11

PMID 28795265

Citations 7

Authors

Tania Tibiletti

Ateeq Ur Rehman

Imre Vass

Christiane Funk

Affiliations

Soon will be listed here.

Abstract

Small CAB-like proteins (SCPs) are single-helix light-harvesting-like proteins found in all organisms performing oxygenic photosynthesis. We investigated the effect of growth in moderate salt stress on these stress-induced proteins in the cyanobacterium Synechocystis sp. PCC 6803 depleted of Photosystem I (PSI), which expresses SCPs constitutively, and compared these cells with a PSI-less/ScpABCDE mutant. SCPs, by stabilizing chlorophyll-binding proteins and Photosystem II (PSII) assembly, protect PSII from photoinhibitory damages, and in their absence electrons accumulate and will lead to ROS formation. The presence of 0.2 M NaCl in the growth medium increased the respiratory activity and other PSII electron sinks in the PSI-less/ScpABCDE strain. We postulate that this salt-induced effect consumes the excess of PSII-generated electrons, reduces the pressure of the electron transport chain, and thereby prevents O production.

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References

Tibiletti T, Hernandez-Prieto M, Matthijs H, Niyogi K, Funk C . Deletion of the gene family of small chlorophyll-binding proteins (ScpABCDE) offsets C/N homeostasis in Synechocystis PCC 6803. Biochim Biophys Acta. 2015; 1857(4):396-407. DOI: 10.1016/j.bbabio.2015.11.011. View

Hill C, Pearson S, Smith A, Rogers L . Inhibition of chlorophyll synthesis in Hordeum vulgare by 3-amino 2,3-dihydrobenzoic acid (gabaculin). Biosci Rep. 1985; 5(9):775-81. DOI: 10.1007/BF01119876. View

Sinha R, Komenda J, Knoppova J, Sedlarova M, Pospisil P . Small CAB-like proteins prevent formation of singlet oxygen in the damaged photosystem II complex of the cyanobacterium Synechocystis sp. PCC 6803. Plant Cell Environ. 2011; 35(4):806-18. DOI: 10.1111/j.1365-3040.2011.02454.x. View

DHaene S, Sobotka R, Bucinska L, Dekker J, Komenda J . Interaction of the PsbH subunit with a chlorophyll bound to histidine 114 of CP47 is responsible for the red 77K fluorescence of Photosystem II. Biochim Biophys Acta. 2015; 1847(10):1327-34. DOI: 10.1016/j.bbabio.2015.07.003. View

Shen G, Boussiba S, Vermaas W . Synechocystis sp PCC 6803 strains lacking photosystem I and phycobilisome function. Plant Cell. 1993; 5(12):1853-63. PMC: 160410. DOI: 10.1105/tpc.5.12.1853. View

Teuber M, Rogner M, Berry S . Fluorescent probes for non-invasive bioenergetic studies of whole cyanobacterial cells. Biochim Biophys Acta. 2001; 1506(1):31-46. DOI: 10.1016/s0005-2728(01)00178-5. View

Shibata M, Katoh H, Sonoda M, Ohkawa H, Shimoyama M, Fukuzawa H . Genes essential to sodium-dependent bicarbonate transport in cyanobacteria: function and phylogenetic analysis. J Biol Chem. 2002; 277(21):18658-64. DOI: 10.1074/jbc.M112468200. View

Lea-Smith D, Ross N, Zori M, Bendall D, Dennis J, Scott S . Thylakoid terminal oxidases are essential for the cyanobacterium Synechocystis sp. PCC 6803 to survive rapidly changing light intensities. Plant Physiol. 2013; 162(1):484-95. PMC: 3641225. DOI: 10.1104/pp.112.210260. View

Cooley J, Vermaas W . Succinate dehydrogenase and other respiratory pathways in thylakoid membranes of Synechocystis sp. strain PCC 6803: capacity comparisons and physiological function. J Bacteriol. 2001; 183(14):4251-8. PMC: 95315. DOI: 10.1128/JB.183.14.4251-4258.2001. View

10.

Storm P, Tibiletti T, Hall M, Funk C . Refolding and enzyme kinetic studies on the ferrochelatase of the cyanobacterium Synechocystis sp. PCC 6803. PLoS One. 2013; 8(2):e55569. PMC: 3563542. DOI: 10.1371/journal.pone.0055569. View

11.

Sonoda M, Katoh H, Vermaas W, Schmetterer G, Ogawa T . Photosynthetic electron transport involved in PxcA-dependent proton extrusion in Synechocystis sp. Strain PCC6803: effect of pxcA inactivation on CO2, HCO3-, and NO3- uptake. J Bacteriol. 1998; 180(15):3799-803. PMC: 107361. DOI: 10.1128/JB.180.15.3799-3803.1998. View

12.

Yao D, Kieselbach T, Komenda J, Promnares K, Prieto M, Tichy M . Localization of the small CAB-like proteins in photosystem II. J Biol Chem. 2006; 282(1):267-76. DOI: 10.1074/jbc.M605463200. View

13.

Sobotka R, Tichy M, Wilde A, Hunter C . Functional assignments for the carboxyl-terminal domains of the ferrochelatase from Synechocystis PCC 6803: the CAB domain plays a regulatory role, and region II is essential for catalysis. Plant Physiol. 2010; 155(4):1735-47. PMC: 3091120. DOI: 10.1104/pp.110.167528. View

14.

Houille-Vernes L, Rappaport F, Wollman F, Alric J, Johnson X . Plastid terminal oxidase 2 (PTOX2) is the major oxidase involved in chlororespiration in Chlamydomonas. Proc Natl Acad Sci U S A. 2011; 108(51):20820-5. PMC: 3251066. DOI: 10.1073/pnas.1110518109. View

15.

Xu H, Vavilin D, Funk C, Vermaas W . Multiple deletions of small Cab-like proteins in the cyanobacterium Synechocystis sp. PCC 6803: consequences for pigment biosynthesis and accumulation. J Biol Chem. 2004; 279(27):27971-9. DOI: 10.1074/jbc.M403307200. View

16.

Promnares K, Komenda J, Bumba L, Nebesarova J, Vacha F, Tichy M . Cyanobacterial small chlorophyll-binding protein ScpD (HliB) is located on the periphery of photosystem II in the vicinity of PsbH and CP47 subunits. J Biol Chem. 2006; 281(43):32705-13. DOI: 10.1074/jbc.M606360200. View

17.

Howitt C, Vermaas W . Quinol and cytochrome oxidases in the cyanobacterium Synechocystis sp. PCC 6803. Biochemistry. 1999; 37(51):17944-51. DOI: 10.1021/bi981486n. View

18.

Kufryk G, Hernandez-Prieto M, Kieselbach T, Miranda H, Vermaas W, Funk C . Association of small CAB-like proteins (SCPs) of Synechocystis sp. PCC 6803 with Photosystem II. Photosynth Res. 2007; 95(2-3):135-45. DOI: 10.1007/s11120-007-9244-3. View

19.

Knoppova J, Sobotka R, Tichy M, Yu J, Konik P, Halada P . Discovery of a chlorophyll binding protein complex involved in the early steps of photosystem II assembly in Synechocystis. Plant Cell. 2014; 26(3):1200-12. PMC: 4001378. DOI: 10.1105/tpc.114.123919. View

20.

Scherer S . Do photosynthetic and respiratory electron transport chains share redox proteins?. Trends Biochem Sci. 1990; 15(12):458-62. DOI: 10.1016/0968-0004(90)90296-n. View