Development of a Red Fluorescent Light-up Probe for Highly Selective and Sensitive Detection of Vicinal Dithiol-containing Proteins in Living Cells
Overview
Affiliations
Vicinal dithiol-containing proteins (VDPs) play a key role in cellular redox homeostasis and are responsible for many diseases. Here, we develop a red fluorescent light-up probe for the highly selective and sensitive detection of VDPs using the environment-sensitive 2-(4-dimethylaminophenyl)-4-(2-carboxyphenyl)-7-diethylamino-1-benzopyrylium () as the fluorescent reporter and cyclic dithiaarsane as the targeting unit. is almost nonfluorescent in aqueous solution. However, it exhibits intense fluorescence emission upon binding to reduced bovine serum albumin (rBSA, selected as the model protein). The fluorescence intensity of is directly proportional to the concentration of rBSA over the range of 0.06-0.9 μM, with a detection limit (3) of 0.015 μM. Importantly, the fast kinetics of binding between and VDPs (∼2.5 min) enables the dynamic tracing of VDPs in biological systems. Preliminary experiments show that can be used for the no-wash imaging of endogenous VDPs in living cells. In addition, our study shows that presents both high environment-sensitivity and good fluorescence properties, and is promising for the development of no-wash fluorescent light-up probes for target-specific proteins in living cells.
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