MiRNA-708 Functions As a Tumour Suppressor in Hepatocellular Carcinoma by Targeting SMAD3

Overview

Journal Oncol Lett

Specialty Oncology

Date 2017 Aug 10

PMID 28789462

Citations 22

Authors

Qi Li

Sheng Li

Yaolu Wu

Feng Gao

Affiliations

Soon will be listed here.

Abstract

Hepatocellular carcinoma (HCC) is the most frequent subtype of primary liver cancer and the third most common cause of cancer-associated mortality worldwide. Previous studies have reported that microRNAs (miRNAs) serve key roles in the carcinogenesis and progression of HCC by regulating gene expression. The present study investigated the expression patterns, biological roles and underlying mechanisms of miRNA-708 (miR-708) in HCC. The expression levels of miR-708 in HCC tissue samples and cell lines were examined. Cell proliferation, migration and invasion assays were used to evaluate the effect of miR-708 on HCC cells. In addition, bioinformatic and western blotting analyses, and dual luciferase reporter assays were performed to investigate the direct gene target of miR-708. The results of the present study demonstrated that miR-708 expression was significantly decreased in HCC tissue samples and cell lines. In addition, the expression level of miR-708 was associated with increased HCC tumour stage. Furthermore, ectopic expression of miR-708 suppressed HCC cell proliferation, migration and invasion. The results of the present study also indicated that miR-708 targets SMAD family member 3 directly . The results of the present study indicated that miR-708 may be a novel target for future HCC therapy.

Citing Articles

MicroRNAs in Hepatocellular Carcinoma Pathogenesis: Insights into Mechanisms and Therapeutic Opportunities.

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MicroRNA as Key Players in Hepatocellular Carcinoma: Insights into Their Role in Metastasis.

Saadh M, Hussain Q, Alazzawi T, Fahdil A, Athab Z, Yarmukhamedov B Biochem Genet. 2024; .

PMID: 39103713 DOI: 10.1007/s10528-024-10897-0.

The Role of TGF-β/SMAD Signaling in Hepatocellular Carcinoma: from Mechanism to Therapy and Prognosis.

Xin X, Cheng X, Zeng F, Xu Q, Hou L Int J Biol Sci. 2024; 20(4):1436-1451.

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