Formation of a Nucleoplasmic Reticulum Requires De Novo Assembly of Nascent Phospholipids and Shows Preferential Incorporation of Nascent Lamins

Overview

Journal Sci Rep

Specialty Science

Date 2017 Aug 9

PMID 28785031

Citations 19

Authors

Marek M Drozdz

Haibo Jiang

Lior Pytowski

Chris Grovenor

David J Vaux

Affiliations

Soon will be listed here.

Abstract

Structure of interphase cell nuclei remains dynamic and can undergo various changes of shape and organisation, in health and disease. The double-membraned envelope that separates nuclear genetic material from the rest of the cell frequently includes deep, branching tubular invaginations that form a dynamic nucleoplasmic reticulum (NR). This study addresses mechanisms by which NR can form in interphase nuclei. We present a combination of Nanoscale Secondary Ion Mass Spectrometry (NanoSIMS) approach and light microscopy techniques to follow formation of NR by using pulse-chase experiments to examine protein and lipid delivery to nascent NR in cultured cells. Lamina protein incorporation was assessed using precursor accumulation (for lamin A) or a MAPLE3 photoconvertible tag (for lamin B1) and membrane phospholipid incorporation using stable isotope labelling with deuterated precursors followed by high resolution NanoSIMS. In all three cases, nascent molecules were selectively incorporated into newly forming NR tubules; thus strongly suggesting that NR formation is a regulated process involving a focal assembly machine, rather than simple physical perturbation of a pre-existing nuclear envelope.

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