Optimization of Supercritical Fluid Extraction of Total Alkaloids, Peimisine, Peimine and Peiminine from the Bulb of Fritillaria Thunbergii Miq, and Evaluation of Antioxidant Activities of the Extracts

Overview

Journal Materials (Basel)

Publisher MDPI

Date 2017 Aug 5

PMID 28773648

Citations 12

Authors

Xiao Ruan

Li Yang

Wen-Xia Cui

Men-Xing Zhang

Zhao-Hui Li

Ben Liu

Qiang Wang

Affiliations

Soon will be listed here.

Abstract

Supercritical fluid extraction (SFE) was used to extract total alkaloids, peimisine, peimine and peiminine from the bulb of Miq. The antioxidant capacity of the extracts was evaluated by DPPH radical scavenging activity (DPPH-RSA), ABTS radical scavenging activity (ABTS-RSA) and ferric reducing capacity (FRAP) assay. A central composite design (CCD) with four variables and five levels was employed for optimization of process parameters, and response surface plots were constructed in accordance with a second order polynomial model. Under optimal conditions of 3.0 h, 60.4 °C, 26.5 MPa and 89.3% ethanol, the highest yields were predicted to be 3.8 mg/g for total alkaloids, 0.5 mg/g for peimisine, 1.3 mg/g for peimine and 1.3 mg/g for peiminine, and the antioxidant capacity of extracts displayed EC value of 5.5 mg/mL, EC value of 0.3 mg/mL and FRAP value of 118.2 mg ascorbic acid equivalent (AAE)/100 g.

Citing Articles

Investigating Changes in Pharmacokinetics of Steroidal Alkaloids from a Hydroethanolic Bulbus Extract in 2,4-Dinitrobenzene Sulfonic Acid-Induced Colitis Rats.

Jeong J, Kim J, Kim J, Chung E, Ko J, Hwang Y Pharmaceuticals (Basel). 2024; 17(8).

PMID: 39204106 PMC: 11357484. DOI: 10.3390/ph17081001.

A distinction between Fritillaria Cirrhosa Bulbus and Fritillaria Pallidiflora Bulbus via LC-MS/MS in conjunction with principal component analysis and hierarchical cluster analysis.

Liu C, Liu S, Tse W, Tse K, Erbu A, Xiong H Sci Rep. 2023; 13(1):2735.

PMID: 36792675 PMC: 9931167. DOI: 10.1038/s41598-023-29631-8.

Efficacy, chemical composition, and pharmacological effects of herbal drugs derived from D. Don and Miq.

Wu F, Tian M, Sun Y, Wu C, Liu X Front Pharmacol. 2022; 13:985935.

PMID: 36532788 PMC: 9748432. DOI: 10.3389/fphar.2022.985935.

Peiminine inhibits myocardial injury and fibrosis after myocardial infarction in rats by regulating mitogen-activated protein kinase pathway.

Chen P, Zhou D, Liu Y, Wang P, Wang W Korean J Physiol Pharmacol. 2022; 26(2):87-94.

PMID: 35203059 PMC: 8890941. DOI: 10.4196/kjpp.2022.26.2.87.

Peiminine Suppresses RANKL-Induced Osteoclastogenesis by Inhibiting the NFATc1, ERK, and NF-κB Signaling Pathways.

Zhu M, Xu W, Jiang J, Wang Y, Guo Y, Yang R Front Endocrinol (Lausanne). 2021; 12:736863.

PMID: 34630331 PMC: 8498341. DOI: 10.3389/fendo.2021.736863.

References

Liu B, Jiang H, Shen B, Chang Y . Supercritical fluid extraction of sinomenine from Sinomenium acutum (Thumb) Rehd et Wils. J Chromatogr A. 2005; 1075(1-2):213-5. DOI: 10.1016/j.chroma.2005.04.016. View

Ling J, Zhang G, Cui Z, Zhang C . Supercritical fluid extraction of quinolizidine alkaloids from Sophora flavescens Ait. and purification by high-speed counter-current chromatography. J Chromatogr A. 2007; 1145(1-2):123-7. DOI: 10.1016/j.chroma.2007.01.080. View

Li D, Zhao J, Li S . High-performance liquid chromatography coupled with post-column dual-bioactivity assay for simultaneous screening of xanthine oxidase inhibitors and free radical scavengers from complex mixture. J Chromatogr A. 2014; 1345:50-6. DOI: 10.1016/j.chroma.2014.03.065. View

Zhang B, Deng Z, Ramdath D, Tang Y, Chen P, Liu R . Phenolic profiles of 20 Canadian lentil cultivars and their contribution to antioxidant activity and inhibitory effects on α-glucosidase and pancreatic lipase. Food Chem. 2014; 172:862-72. DOI: 10.1016/j.foodchem.2014.09.144. View

Shirwaikar A, Shirwaikar A, Rajendran K, Punitha I . In vitro antioxidant studies on the benzyl tetra isoquinoline alkaloid berberine. Biol Pharm Bull. 2006; 29(9):1906-10. DOI: 10.1248/bpb.29.1906. View

Reiter R, Tan D, Fuentes-Broto L . Melatonin: a multitasking molecule. Prog Brain Res. 2010; 181:127-51. DOI: 10.1016/S0079-6123(08)81008-4. View

Herraiz T, Galisteo J . Hydroxyl radical reactions and the radical scavenging activity of β-carboline alkaloids. Food Chem. 2014; 172:640-9. DOI: 10.1016/j.foodchem.2014.09.091. View

Temel E, Alasalvar C, Gokce H, Guder A, Albayrak C, Alpaslan Y . DFT calculations, spectroscopy and antioxidant activity studies on (E)-2-nitro-4-[(phenylimino)methyl]phenol. Spectrochim Acta A Mol Biomol Spectrosc. 2014; 136 Pt B:534-46. DOI: 10.1016/j.saa.2014.09.067. View

Xu Q, Shen Y, Wang H, Zhang N, Xu S, Zhang L . Application of response surface methodology to optimise extraction of flavonoids from fructus sophorae. Food Chem. 2013; 138(4):2122-9. DOI: 10.1016/j.foodchem.2012.11.099. View

10.

Dapkevicius A, Van Beek T, Niederlander H . Evaluation and comparison of two improved techniques for the on-line detection of antioxidants in liquid chromatography eluates. J Chromatogr A. 2001; 912(1):73-82. DOI: 10.1016/s0021-9673(01)00548-9. View

11.

Kou X, Chen Q, Li X, Li M, Kan C, Chen B . Quantitative assessment of bioactive compounds and the antioxidant activity of 15 jujube cultivars. Food Chem. 2014; 173:1037-44. DOI: 10.1016/j.foodchem.2014.10.110. View

12.

Helmja K, Vaher M, Pussa T, Kaljurand M . Analysis of the stable free radical scavenging capability of artificial polyphenol mixtures and plant extracts by capillary electrophoresis and liquid chromatography-diode array detection-tandem mass spectrometry. J Chromatogr A. 2009; 1216(12):2417-23. DOI: 10.1016/j.chroma.2009.01.040. View

13.

Herrero M, Mendiola J, Cifuentes A, Ibanez E . Supercritical fluid extraction: Recent advances and applications. J Chromatogr A. 2009; 1217(16):2495-511. DOI: 10.1016/j.chroma.2009.12.019. View

14.

Choi Y, Chin Y, Kim J, Jeon S, Yoo K . Strategies for supercritical fluid extraction of hyoscyamine and scopolamine salts using basified modifiers. J Chromatogr A. 1999; 863(1):47-55. DOI: 10.1016/s0021-9673(99)00962-0. View

15.

Li Y, Chen J, Li Y, Li Q, Zheng Y, Fu Y . Screening and characterization of natural antioxidants in four Glycyrrhiza species by liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry. J Chromatogr A. 2011; 1218(45):8181-91. DOI: 10.1016/j.chroma.2011.09.030. View

16.

Raudonis R, Raudone L, Jakstas V, Janulis V . Comparative evaluation of post-column free radical scavenging and ferric reducing antioxidant power assays for screening of antioxidants in strawberries. J Chromatogr A. 2012; 1233:8-15. DOI: 10.1016/j.chroma.2012.02.019. View

17.

Tsoi B, Yi R, Cao L, Li S, Tan R, Chen M . Comparing antioxidant capacity of purine alkaloids: a new, efficient trio for screening and discovering potential antioxidants in vitro and in vivo. Food Chem. 2015; 176:411-9. DOI: 10.1016/j.foodchem.2014.12.087. View

18.

Paz M, Gullon P, Barroso M, Carvalho A, Domingues V, Gomes A . Brazilian fruit pulps as functional foods and additives: evaluation of bioactive compounds. Food Chem. 2014; 172:462-8. DOI: 10.1016/j.foodchem.2014.09.102. View

19.

Hao D, Gu X, Xiao P, Peng Y . Phytochemical and biological research of Fritillaria medicine resources. Chin J Nat Med. 2013; 11(4):330-44. DOI: 10.1016/S1875-5364(13)60050-3. View

20.

Zheng Y, Liu B, Chen M, Chen T . Supercritical fluid extraction of ecdysterone from the roots of Achyranthes bidentata BL. J Sep Sci. 2008; 31(8):1393-8. DOI: 10.1002/jssc.200700468. View