PolystyreneDivinylbenzene PolyHIPE Monoliths in 1.0 Mm Column Formats for Liquid Chromatography

Overview

Journal Materials (Basel)

Publisher MDPI

Date 2017 Aug 5

PMID 28773337

Citations 4

Authors

Sidratul Choudhury

Laurence Fitzhenry

Blanaid White

Damian Connolly

Affiliations

Soon will be listed here.

Abstract

The reversed phase liquid chromatographic (RP-HPLC) separation of small molecules using a polystyrene--divinylbenzene (PSDVB) polyHIPE stationary phases housed within 1.0 mm i.d. silcosteel columns is presented within this study. A 90% PSDVB polyHIPE was covalently attached to the walls of the column housing by prior wall modification with 3-(trimethoxysilyl) propyl methacrylate and could withstand operating backpressures in excess of 200 bar at a flow rate of 1.2 mL/min. Permeability studies revealed that the monolith swelled slightly in 100% acetonitrile relative to 100% water but could nevertheless be used to separate five alkylbenzenes using a flow rate of 40 µL/min (linear velocity: 0.57 mm/s). Remarkable column-to-column reproducibility is shown with retention factor variation between 2.6% and 6.1% for two separately prepared columns.

Citing Articles

Effect of shearing stress on the radial heterogeneity and chromatographic performance of styrene-based polymerised high internal phase emulsions prepared in capillary format.

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