Effect of Different Carbon Sources on Bacterial Nanocellulose Production and Structure Using the Low PH Resistant Strain Komagataeibacter Medellinensis

Overview

Journal Materials (Basel)

Publisher MDPI

Date 2017 Aug 5

PMID 28773001

Citations 25

Authors

Carlos Molina-Ramirez

Margarita Castro

Marlon Osorio

Mabel Torres-Taborda

Beatriz Gomez

Robin Zuluaga

Catalina Gomez

Piedad Ganan

Orlando J Rojas

Cristina Castro

Affiliations

Soon will be listed here.

Abstract

Bacterial cellulose (BC) is a polymer obtained by fermentation with microorganism of different genera. Recently, new producer species have been discovered, which require identification of the most important variables affecting cellulose production. In this work, the influence of different carbon sources in BC production by a novel low pH-resistant strain was established. The Hestrin-Schramm culture medium was used as a reference and was compared to other media comprising glucose, fructose, and sucrose, used as carbon sources at three concentrations (1, 2, and 3% /). The BC yield and dynamics of carbon consumption were determined at given fermentation times during cellulose production. While the carbon source did not influence the BC structural characteristics, different production levels were determined: glucose > sucrose > fructose. These results highlight considerations to improve BC industrial production and to establish the BC property space for applications in different fields.

Citing Articles

Exploring the evolution of bacterial cellulose precursors and their potential use as cellulose-based building blocks.

Mauro F, Corrado B, De Gregorio V, Lagreca E, Di Natale C, Vecchione R Sci Rep. 2024; 14(1):11613.

PMID: 38773229 PMC: 11109180. DOI: 10.1038/s41598-024-62462-9.

Mucoadhesive capsules based on bacterial nanocellulose and chitosan as delivery system of turmeric extract.

Posada L, Jaramillo-Quiceno N, Castro C, Osorio M Heliyon. 2023; 9(11):e21836.

PMID: 38034640 PMC: 10682617. DOI: 10.1016/j.heliyon.2023.e21836.

as exopolysaccharide producers Current state of knowledge and further perspectives.

Wunsche J, Schmid J Front Bioeng Biotechnol. 2023; 11:1166618.

PMID: 37064223 PMC: 10097950. DOI: 10.3389/fbioe.2023.1166618.

Improved production of bacterial cellulose by Komagataeibacter europaeus employing fruit extract as carbon source.

Tseng Y, Patel A, Chen C, Dong C, Singhania R J Food Sci Technol. 2023; 60(3):1054-1064.

PMID: 36908337 PMC: 9998749. DOI: 10.1007/s13197-022-05451-y.

Bacterial nanocellulose production using Cantaloupe juice, statistical optimization and characterization.

El-Naggar N, Mohammed A, El-Malkey S Sci Rep. 2023; 13(1):51.

PMID: 36593253 PMC: 9807561. DOI: 10.1038/s41598-022-26642-9.

References

Cheng K, Catchmark J, Demirci A . Enhanced production of bacterial cellulose by using a biofilm reactor and its material property analysis. J Biol Eng. 2009; 3:12. PMC: 2724407. DOI: 10.1186/1754-1611-3-12. View

Kim S, Kim J, Wee Y, Park D, Ryu H . Production of bacterial cellulose by Gluconacetobacter sp. RKY5 isolated from persimmon vinegar. Appl Biochem Biotechnol. 2008; 131(1-3):705-15. DOI: 10.1385/ABAB:131:1:705. View

Ogino H, Azuma Y, Hosoyama A, Nakazawa H, Matsutani M, Hasegawa A . Complete genome sequence of NBRC 3288, a unique cellulose-nonproducing strain of Gluconacetobacter xylinus isolated from vinegar. J Bacteriol. 2011; 193(24):6997-8. PMC: 3232855. DOI: 10.1128/JB.06158-11. View

Romling U . Molecular biology of cellulose production in bacteria. Res Microbiol. 2002; 153(4):205-12. DOI: 10.1016/s0923-2508(02)01316-5. View

Jung H, Jeong J, Lee O, Park G, Kim K, Park H . Influence of glycerol on production and structural-physical properties of cellulose from Acetobacter sp. V6 cultured in shake flasks. Bioresour Technol. 2010; 101(10):3602-8. DOI: 10.1016/j.biortech.2009.12.111. View

Castro C, Cleenwerck I, Trcek J, Zuluaga R, de Vos P, Caro G . Gluconacetobacter medellinensis sp. nov., cellulose- and non-cellulose-producing acetic acid bacteria isolated from vinegar. Int J Syst Evol Microbiol. 2012; 63(Pt 3):1119-1125. DOI: 10.1099/ijs.0.043414-0. View

Tahara N, Tabuchi M, Watanabe K, Yano H, Morinaga Y, Yoshinaga F . Degree of Polymerization of Cellulose from Acetobacter xylinum BPR2001 Decreased by Cellulase Produced by the Strain. Biosci Biotechnol Biochem. 2016; 61(11):1862-5. DOI: 10.1271/bbb.61.1862. View

Yamada Y . Transfer of Gluconacetobacter kakiaceti, Gluconacetobacter medellinensis and Gluconacetobacter maltaceti to the genus Komagataeibacter as Komagataeibacter kakiaceti comb. nov., Komagataeibacter medellinensis comb. nov. and Komagataeibacter maltaceti.... Int J Syst Evol Microbiol. 2014; 64(Pt 5):1670-1672. DOI: 10.1099/ijs.0.054494-0. View

Csonka L . Physiological and genetic responses of bacteria to osmotic stress. Microbiol Rev. 1989; 53(1):121-47. PMC: 372720. DOI: 10.1128/mr.53.1.121-147.1989. View

10.

Ross P, Mayer R, Benziman M . Cellulose biosynthesis and function in bacteria. Microbiol Rev. 1991; 55(1):35-58. PMC: 372800. DOI: 10.1128/mr.55.1.35-58.1991. View

11.

Seesuriyachan P, Kuntiya A, Hanmoungjai P, Techapun C, Chaiyaso T, Leksawasdi N . Optimization of exopolysaccharide overproduction by Lactobacillus confusus in solid state fermentation under high salinity stress. Biosci Biotechnol Biochem. 2012; 76(5):912-7. DOI: 10.1271/bbb.110905. View

12.

Mikkelsen D, Flanagan B, Dykes G, Gidley M . Influence of different carbon sources on bacterial cellulose production by Gluconacetobacter xylinus strain ATCC 53524. J Appl Microbiol. 2009; 107(2):576-83. DOI: 10.1111/j.1365-2672.2009.04226.x. View

13.

Max B, Salgado J, Rodriguez N, Cortes S, Converti A, Dominguez J . Biotechnological production of citric acid. Braz J Microbiol. 2013; 41(4):862-75. PMC: 3769771. DOI: 10.1590/S1517-83822010000400005. View

14.

Benziman M, Rivetz B . Factors affecting hexose phosphorylation in Acetobacter xylinum. J Bacteriol. 1972; 111(2):325-33. PMC: 251285. DOI: 10.1128/jb.111.2.325-333.1972. View

15.

Nguyen V, Gidley M, Dykes G . Potential of a nisin-containing bacterial cellulose film to inhibit Listeria monocytogenes on processed meats. Food Microbiol. 2008; 25(3):471-8. DOI: 10.1016/j.fm.2008.01.004. View

16.

Saichana N, Matsushita K, Adachi O, Frebort I, Frebortova J . Acetic acid bacteria: A group of bacteria with versatile biotechnological applications. Biotechnol Adv. 2014; 33(6 Pt 2):1260-71. DOI: 10.1016/j.biotechadv.2014.12.001. View

17.

Castro C, Zuluaga R, Alvarez C, Putaux J, Caro G, Rojas O . Bacterial cellulose produced by a new acid-resistant strain of Gluconacetobacter genus. Carbohydr Polym. 2014; 89(4):1033-7. DOI: 10.1016/j.carbpol.2012.03.045. View

18.

Hutchens S, Leon R, ONeill H, Evans B . Statistical analysis of optimal culture conditions for Gluconacetobacter hansenii cellulose production. Lett Appl Microbiol. 2007; 44(2):175-80. DOI: 10.1111/j.1472-765X.2006.02055.x. View

19.

Ruka D, Simon G, Dean K . Altering the growth conditions of Gluconacetobacter xylinus to maximize the yield of bacterial cellulose. Carbohydr Polym. 2014; 89(2):613-22. DOI: 10.1016/j.carbpol.2012.03.059. View