Effects of Metal Micro and Nano-Particles on HASCs: An In Vitro Model

Overview

Journal Nanomaterials (Basel)

Date 2017 Aug 4

PMID 28771169

Citations 10

Authors

Silvia Palombella

Cristina Pirrone

Federica Rossi

Ilaria Armenia

Mario Cherubino

Luigi Valdatta

Mario Raspanti

Giovanni Bernardini

Rosalba Gornati

Affiliations

Soon will be listed here.

Abstract

As the knowledge about the interferences of nanomaterials on human staminal cells are scarce and contradictory, we undertook a comparative multidisciplinary study based on the size effect of zero-valent iron, cobalt, and nickel microparticles (MPs) and nanoparticles (NPs) using human adipose stem cells (hASCs) as a model, and evaluating cytotoxicity, morphology, cellular uptake, and gene expression. Our results suggested that the medium did not influence the cell sensitivity but, surprisingly, the iron microparticles (FeMPs) resulted in being toxic. These data were supported by modifications in mRNA expression of some genes implicated in the inflammatory response. Microscopic analysis confirmed that NPs, mainly internalized by endocytosis, persist in the vesicles without any apparent cell damage. Conversely, MPs are not internalized, and the effects on hASCs have to be ascribed to the release of ions in the culture medium, or to the reduced oxygen and nutrient exchange efficiency due to the presence of MP agglomerating around the cells. Notwithstanding the results depicting a heterogeneous scene that does not allow drawing a general conclusion, this work reiterates the importance of comparative investigations on MPs, NPs, and corresponding ions, and the need to continue the thorough verification of NP and MP innocuousness to ensure unaffected stem cell physiology and differentiation.

Citing Articles

The pulmonary effects of nickel-containing nanoparticles: Cytotoxicity, genotoxicity, carcinogenicity, and their underlying mechanisms.

Mo Y, Zhang Y, Zhang Q Environ Sci Nano. 2024; 11(5):1817-1846.

PMID: 38984270 PMC: 11230653. DOI: 10.1039/d3en00929g.

Toxicological assessment of nanocrystalline metal alloys with potential applications in the aeronautical field.

Rumbo C, Bianchin A, Locci A, Barros R, Martel Martin S, Tamayo-Ramos J Sci Rep. 2022; 12(1):1523.

PMID: 35087098 PMC: 8795356. DOI: 10.1038/s41598-022-05406-5.

In vitro safety evaluation of rare earth-lean alloys for permanent magnets manufacturing.

Rumbo C, Espina C, Gassmann J, Tosoni O, Barros Garcia R, Martel Martin S Sci Rep. 2021; 11(1):12633.

PMID: 34135388 PMC: 8209147. DOI: 10.1038/s41598-021-91890-0.

Human Platelet Lysate Acts Synergistically With Laminin to Improve the Neurotrophic Effect of Human Adipose-Derived Stem Cells on Primary Neurons .

Guiotto M, Raffoul W, Hart A, Riehle M, di Summa P Front Bioeng Biotechnol. 2021; 9:658176.

PMID: 33816456 PMC: 8017201. DOI: 10.3389/fbioe.2021.658176.

Biological Roles and Delivery Strategies for Ions to Promote Osteogenic Induction.

Bosch-Rue E, Diez-Tercero L, Giordano-Kelhoffer B, Delgado L, Bosch B, Hoyos-Nogues M Front Cell Dev Biol. 2021; 8:614545.

PMID: 33520992 PMC: 7841204. DOI: 10.3389/fcell.2020.614545.

References

Shi J, Wang B, Chen Z, Liu W, Pan J, Hou L . A Multi-Functional Tumor Theranostic Nanoplatform for MRI Guided Photothermal-Chemotherapy. Pharm Res. 2016; 33(6):1472-85. DOI: 10.1007/s11095-016-1891-7. View

Rancoule C, Magne N, Vallard A, Guy J, Rodriguez-Lafrasse C, Deutsch E . Nanoparticles in radiation oncology: From bench-side to bedside. Cancer Lett. 2016; 375(2):256-262. DOI: 10.1016/j.canlet.2016.03.011. View

Lacroix L, Delpech F, Nayral C, Lachaize S, Chaudret B . New generation of magnetic and luminescent nanoparticles for in vivo real-time imaging. Interface Focus. 2014; 3(3):20120103. PMC: 3638419. DOI: 10.1098/rsfs.2012.0103. View

Rudge S, Kurtz T, Vessely C, Catterall L, Williamson D . Preparation, characterization, and performance of magnetic iron-carbon composite microparticles for chemotherapy. Biomaterials. 2000; 21(14):1411-20. DOI: 10.1016/s0142-9612(00)00006-5. View

Klimczak A, Kozlowska U . Mesenchymal Stromal Cells and Tissue-Specific Progenitor Cells: Their Role in Tissue Homeostasis. Stem Cells Int. 2016; 2016:4285215. PMC: 4707334. DOI: 10.1155/2016/4285215. View

Cherubino M, Valdatta L, Balzaretti R, Pellegatta I, Rossi F, Protasoni M . Human adipose-derived stem cells promote vascularization of collagen-based scaffolds transplanted into nude mice. Regen Med. 2016; 11(3):261-71. PMC: 4976995. DOI: 10.2217/rme-2015-0010. View

Lim D, Jang J, Kim S, Kang T, Lee K, Choi I . The effects of sub-lethal concentrations of silver nanoparticles on inflammatory and stress genes in human macrophages using cDNA microarray analysis. Biomaterials. 2012; 33(18):4690-9. DOI: 10.1016/j.biomaterials.2012.03.006. View

Cattaneo A, Gornati R, Sabbioni E, Chiriva-Internati M, Cobos E, Jenkins M . Nanotechnology and human health: risks and benefits. J Appl Toxicol. 2010; 30(8):730-44. DOI: 10.1002/jat.1609. View

Tan Y, Best S, Donnelly C, Olweny E, Kapur P, Mir S . Novel iron oxide microparticles used to render stone fragments paramagnetic: assessment of toxicity in a murine model. J Urol. 2012; 188(5):1972-7. DOI: 10.1016/j.juro.2012.07.005. View

10.

Gnecchi M, Zhang Z, Ni A, Dzau V . Paracrine mechanisms in adult stem cell signaling and therapy. Circ Res. 2008; 103(11):1204-19. PMC: 2667788. DOI: 10.1161/CIRCRESAHA.108.176826. View

11.

Papis E, Rossi F, Raspanti M, Dalle-Donne I, Colombo G, Milzani A . Engineered cobalt oxide nanoparticles readily enter cells. Toxicol Lett. 2009; 189(3):253-9. DOI: 10.1016/j.toxlet.2009.06.851. View

12.

Simpson R, Hammacher A, Smith D, Matthews J, Ward L . Interleukin-6: structure-function relationships. Protein Sci. 1997; 6(5):929-55. PMC: 2143693. DOI: 10.1002/pro.5560060501. View

13.

Macro L, Jaiswal J, Simon S . Dynamics of clathrin-mediated endocytosis and its requirement for organelle biogenesis in Dictyostelium. J Cell Sci. 2012; 125(Pt 23):5721-32. PMC: 3575707. DOI: 10.1242/jcs.108837. View

14.

Gornati R, Pedretti E, Rossi F, Cappellini F, Zanella M, Olivato I . Zerovalent Fe, Co and Ni nanoparticle toxicity evaluated on SKOV-3 and U87 cell lines. J Appl Toxicol. 2015; 36(3):385-93. PMC: 5054872. DOI: 10.1002/jat.3220. View

15.

Bardack S, Dalgard C, Kalinich J, Kasper C . Genotoxic changes to rodent cells exposed in vitro to tungsten, nickel, cobalt and iron. Int J Environ Res Public Health. 2014; 11(3):2922-40. PMC: 3987013. DOI: 10.3390/ijerph110302922. View

16.

Ahamed M . Toxic response of nickel nanoparticles in human lung epithelial A549 cells. Toxicol In Vitro. 2011; 25(4):930-6. DOI: 10.1016/j.tiv.2011.02.015. View

17.

Petrarca C, Clemente E, Amato V, Pedata P, Sabbioni E, Bernardini G . Engineered metal based nanoparticles and innate immunity. Clin Mol Allergy. 2015; 13(1):13. PMC: 4503298. DOI: 10.1186/s12948-015-0020-1. View

18.

Hackenberg S, Scherzed A, Kessler M, Hummel S, Technau A, Froelich K . Silver nanoparticles: evaluation of DNA damage, toxicity and functional impairment in human mesenchymal stem cells. Toxicol Lett. 2010; 201(1):27-33. DOI: 10.1016/j.toxlet.2010.12.001. View

19.

Lai J, Lai M, Jandhyam S, Dukhande V, Bhushan A, Daniels C . Exposure to titanium dioxide and other metallic oxide nanoparticles induces cytotoxicity on human neural cells and fibroblasts. Int J Nanomedicine. 2009; 3(4):533-45. PMC: 2636591. DOI: 10.2147/ijn.s3234. View

20.

Gronthos S, Zannettino A . Methods for the purification and characterization of human adipose-derived stem cells. Methods Mol Biol. 2010; 702:109-20. DOI: 10.1007/978-1-61737-960-4_9. View